TRANSGENIC PLANT EVENT DETECTION
First Claim
Patent Images
1. A method to examine a sample for the presence or absence of material derived from one or more transgenic plant events comprising the steps of:
- (1) detecting the presence or absence in the sample of nucleic acids comprising;
one, more than one or all of nucleic acids chosen from;
a) “
Zm”
;
a nucleic acid derived from and specific for Zea mays taxon, preferably Zea mays ssp. mays, b) “
Bn”
;
a nucleic acid derived from and specific for Brassica napus taxon,c) “
Gm”
;
a nucleic acid derived from and specific for Glycine max taxon,o) “
Or”
;
a nucleic acid derived from and specific for Oryza sativa taxon,p) “
By”
;
a nucleic acid derived from and specific for Beta vulgaris taxon,q) “
Gs”
;
a nucleic acid derived from and specific for Gossypium taxon, andt) “
St”
;
a nucleic acid derived from and specific for Solanum tuberosum taxon; and
one, more than one or all of nucleic acids chosen from;
d) “
p35S”
;
a nucleic acid derived from the 35S promoter of Cauliflower Mosaic Virus,e) “
tNOS”
;
a nucleic acid derived from the 3′
terminator of the Agrobacterium tumefaciens nopaline synthetase gene,f) “
Cry1Ab”
;
a nucleic acid derived from the crystal protein gene Cry1Ab of Bacillus thuringiensis, g) “
PAT/bar”
;
a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene bar of Streptomyces hygroscopicus, h) “
PAT/pat”
;
a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene pat of Streptomyces viridochromogenes, andi) “
CP4-EPSPS”
;
a nucleic acid derived from the 5-Enol-pyruvylshikimate-3-phosphate synthase EPSPS gene from Agrobacterium sp. CP4; and
(2) concluding the presence or absence in the sample of material derived from one or more transgenic plant events chosen from the group comprising;
events Bt176, Bt11, Bt10, MON810, MON863, TC1507, NK603, T25, GA21, DAS-59122, MIR604, LY038, MON88017, crosses thereof, and related events thereof;
events Topas 19/2, MS1, RF1, RF2, RF3, MS8, GT73, T45, Liberator pHoe6/Ac, GS40/90pHoe6/Ac, OXY235, crosses thereof including MS1/RF1, MS1/RF2, MS8/RF3, and related events thereof;
events MON 40-3-2, MON89788, A2704-12, A5547-127, crosses thereof, and related events thereof, events LL62, LL06 and LL601, crosses thereof, and related events thereof;
events T120-7, H7-1 and A5-15, crosses thereof, and related events thereof;
events LL cotton 25, MON 1445, MON 531, MON15985, crosses thereof, and related events thereof; and
event EH92-527-1 and related events thereof,wherein in step (1) the presence or absence of nucleic acids in a sample is detected using PCR amplification, preferably real-time PCR amplification, using the respective primer pairs from the following table, or variants or derivatives of said primer pairs;
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Abstract
The present invention relates to detection of materials derived from transgenic plant events. In particular, the invention provides methods, reagents, kits and reference materials for detecting the presence or absence in a sample of genetic material derived from and attributable to select transgenic plant events.
9 Citations
27 Claims
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1. A method to examine a sample for the presence or absence of material derived from one or more transgenic plant events comprising the steps of:
-
(1) detecting the presence or absence in the sample of nucleic acids comprising; one, more than one or all of nucleic acids chosen from; a) “
Zm”
;
a nucleic acid derived from and specific for Zea mays taxon, preferably Zea mays ssp. mays,b) “
Bn”
;
a nucleic acid derived from and specific for Brassica napus taxon,c) “
Gm”
;
a nucleic acid derived from and specific for Glycine max taxon,o) “
Or”
;
a nucleic acid derived from and specific for Oryza sativa taxon,p) “
By”
;
a nucleic acid derived from and specific for Beta vulgaris taxon,q) “
Gs”
;
a nucleic acid derived from and specific for Gossypium taxon, andt) “
St”
;
a nucleic acid derived from and specific for Solanum tuberosum taxon; andone, more than one or all of nucleic acids chosen from; d) “
p35S”
;
a nucleic acid derived from the 35S promoter of Cauliflower Mosaic Virus,e) “
tNOS”
;
a nucleic acid derived from the 3′
terminator of the Agrobacterium tumefaciens nopaline synthetase gene,f) “
Cry1Ab”
;
a nucleic acid derived from the crystal protein gene Cry1Ab of Bacillus thuringiensis,g) “
PAT/bar”
;
a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene bar of Streptomyces hygroscopicus,h) “
PAT/pat”
;
a nucleic acid derived from the phosphinothricin acetyltransferase (PAT) gene pat of Streptomyces viridochromogenes, andi) “
CP4-EPSPS”
;
a nucleic acid derived from the 5-Enol-pyruvylshikimate-3-phosphate synthase EPSPS gene from Agrobacterium sp. CP4; and(2) concluding the presence or absence in the sample of material derived from one or more transgenic plant events chosen from the group comprising;
events Bt176, Bt11, Bt10, MON810, MON863, TC1507, NK603, T25, GA21, DAS-59122, MIR604, LY038, MON88017, crosses thereof, and related events thereof;
events Topas 19/2, MS1, RF1, RF2, RF3, MS8, GT73, T45, Liberator pHoe6/Ac, GS40/90pHoe6/Ac, OXY235, crosses thereof including MS1/RF1, MS1/RF2, MS8/RF3, and related events thereof;
events MON 40-3-2, MON89788, A2704-12, A5547-127, crosses thereof, and related events thereof, events LL62, LL06 and LL601, crosses thereof, and related events thereof;
events T120-7, H7-1 and A5-15, crosses thereof, and related events thereof;
events LL cotton 25, MON 1445, MON 531, MON15985, crosses thereof, and related events thereof; and
event EH92-527-1 and related events thereof,wherein in step (1) the presence or absence of nucleic acids in a sample is detected using PCR amplification, preferably real-time PCR amplification, using the respective primer pairs from the following table, or variants or derivatives of said primer pairs; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 23, 24, 25, 26, 27)
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- 20. Recombinant E. coli as deposited under the Budapest Treaty with the Belgian Coordinated Collections of Microorganisms (BCCM) on Jan. 10, 2007 under LMBP accession numbers LMBP 5452, LMBP 5453, LMBP 5454, LMBP 5455, LMBP 5456, LMBP 5457, LMBP 5458, LMBP 5459 and LMBP 5460, on Mar. 6, 2007 under LMBP accession number LMBP 5451, and on Apr. 19, 2007 under LMBP accession numbers LMBP 5587, LMBP 5588, LMBP 5589 and LMBP 5590.
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Current AssigneeSciensano Co.
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Original AssigneeScientific Institute of Public Health
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InventorsBarbau-Piednoir, Elodie, Lievens, Antoon Piet Nelly Raoul, Van Den Bulcke, Marc Henri Germain, Sneyers, Myriam Jacqueline Sylviane, Mbongolo Mbella, Etondoh Guillaume, Leunda, Amaya
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Granted Patent
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Time in Patent OfficeDays
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Field of Search
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US Class Current435/6
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CPC Class CodesC12Q 1/6844 Nucleic acid amplification ...C12Q 1/6895 for plants, fungi or algaeC12Q 2537/143 Multiplexing, i.e. use of m...C12Q 2600/16 Primer sets for multiplex a...Y02A 40/146 Genetically Modified [GMO] ...
