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METHYLATION ANALYSIS OF MATE PAIRS

  • US 20100120034A1
  • Filed: 07/06/2009
  • Published: 05/13/2010
  • Est. Priority Date: 07/03/2008
  • Status: Abandoned Application
First Claim
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1. A method of analyzing the methylation state of genomic DNA, comprising:

  • fragmenting a genomic DNA sample, whereby genomic DNA fragments are produced;

    circularizing a genomic DNA fragment to produce a double-stranded circular DNA comprising a nick on at least one strand of the double-stranded circular DNA;

    linearizing the circular DNA;

    adding a nick translation enzyme in the presence of methylation conversion agent resistant nucleotide triphosphates, whereby a partially methylation conversion agent resistant polynucleotide is generated, wherein the partially methylation conversion agent resistant polynucleotide has a tag region that is methylation conversion agent resistant and a tag region that is not methylation conversion agent resistant.

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