SEQUENTIAL ANALYSIS OF BIOLOGICAL SAMPLES
First Claim
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1. A method of detecting a plurality of targets in a biological sample, comprising:
- (a) contacting the biological sample with a plurality of target-binding probes to form a plurality of target-bound probes;
(b) observing a first set of signals from a first set of the plurality of target-bound probes;
(c) modifying the observed signals;
(d) generating a second set of signals from a second set of the plurality of target-bound probes; and
(e) observing the second set of signals.
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Abstract
Methods for detecting a plurality of targets in a biological sample are provided. The method comprises contacting the biological sample with a plurality of target-binding probes simultaneously to form a plurality of target-bound probes and observing the signals from the target-bound probes sequentially. An associated kit and device for detection of the plurality of targets are also provided.
135 Citations
32 Claims
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1. A method of detecting a plurality of targets in a biological sample, comprising:
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(a) contacting the biological sample with a plurality of target-binding probes to form a plurality of target-bound probes; (b) observing a first set of signals from a first set of the plurality of target-bound probes; (c) modifying the observed signals; (d) generating a second set of signals from a second set of the plurality of target-bound probes; and (e) observing the second set of signals. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A method of detecting a plurality of targets in a biological sample, comprising:
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(a) contacting the biological sample with a plurality of target-binding probes to form a plurality of target-bound probes; (b) generating a first set of signals from a first set of the plurality of target-bound probes; (c) observing the first set of signals; (d) modifying the observed signals; (e) generating a second set of signals from a second set of the plurality of target-bound probes; and (f) observing the second set of signals. - View Dependent Claims (19, 20, 21, 22, 23)
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24. A method of detecting a plurality of targets in a biological sample, comprising:
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(a) contacting the sample with a plurality of target-binding probes to form a plurality of target-bound probes; (b) contacting the plurality of target-bound probes with a first set signal-generating probes that are capable of binding a first set of the plurality of target-bound probes, and are capable of generating a first set of signals; (c) detecting the first set of the plurality of targets via observing the first set of signals; (d) modifying the observed first set of signals; and (e) repeating steps (b) to (d) multiple times using a subsequent set of signal-generating probes to detect a subsequent set of the plurality of targets, wherein the subsequent set of signal-generating probes are capable of binding a subsequent set of the plurality of target-bound probes, and are capable of generating a subsequent set of signals. - View Dependent Claims (25, 26, 27, 28, 29)
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30. A device for detection of a plurality of targets in a biological sample, comprising:
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a sample handling system; a reagent dispensing system; and a signal detection system, wherein the device is operable to detect the plurality of targets in the biological sample using a method comprising the steps of; (a) contacting the biological sample with a plurality of target-binding probes to form a plurality of target-bound probes; (b) observing a first set of signals from a first set of the plurality of target-bound probes; (c) modifying the observed signals; (d) generating a second set of signals from a second set of the plurality of target-bound probes; and (e) observing the second set of signals. - View Dependent Claims (31, 32)
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Specification