Process for typing of HCV isolates

• US 20100120121A1
• Filed: 07/12/2007
• Published: 05/13/2010
• Est. Priority Date: 11/27/1992
• Status: Active Grant

• Current Assignee: Innogenetics NV (H.U. Group Holdings, Inc.)
• Original Assignee: Innogenetics NV (H.U. Group Holdings, Inc.)
• Inventors: Maertens, Geert, Stuyver, Lieven, Van Heuverswyn, Hugo, Rossau, Rudi
• Granted Patent: US 8,101,351 B2
• Time in Patent Office: Days
• US Class Current: 435/235.100
• CPC Class Codes: C12Q 1/707 non-A, non-B Hepatitis, excluding hepatitis D

Abstract

The invention relates to a process for genotyping any HCV isolate present in a biological sample, previously identified as being HCV positive, and for classifying said isolate according to the percentage of homology with other HCV isolates, comprising the steps of:

• • contacting said sample in which the ribonucleotides or deoxyribonucleotides have been made accessible, if need be, under suitable denaturation, with at least one probe from about 10 to about 40 nucleotides, with said probe being liable to hybridize to a region being in the domain extending from nucleotide at position −291 to nucleotide at position −66 of the 5′ untranslated region of one of the HCV isolates represented by their cDNA sequences, with said numbering of position beginning with the first ATG codon of the open reading frame encoding the HCV polyprotein, or with said probe being complementary to the above-defined probes,
  • detecting the complexes possibly formed between said probe and the nucleotide sequence of the HCV isolate to be identified.

Claims

1. An isolated polynucleic acid specifically hybridizing with a target sequence selected from the group consisting of a nucleic acid sequence encoding any of the NS5B amino acid sequences defined by SEQ ID NO:

82 to SEQ ID NO;

92, or the complement thereof.

2. The isolated polynucleic acid of claim 1 hybridizing under conditions allowing detection of homologous target sequences comprising one or few mismatches.

3. The isolated polynucleic acid of claim 1 hybridizing under conditions allowing detection of completely homologous target sequences.

4. The isolated polynucleic acid of claim 1 comprising 10 to 50 contiguous nucleotides of a nucleic acid sequence encoding any of said NS5B amino acid sequences or the complement thereof.

5. The isolated polynucleic acid of claim 1 which is a capture probe or a labeled probe and which is optionally immobilized to a solid substrate.

7. A kit for detecting or typing HCV isolates comprising at least one isolated polynucleic acid of claim 1.

6. An isolated HCV virus characterized by any of the HCV NS5 nucleotide sequences selected from the group consisting of a nucleic acid sequence encoding any of SEQ ID NO:

82 to SEQ ID NO;

92.