Pluripotent Stem Cell Culture on Micro-Carriers
First Claim
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1. A method for the propagation of pluripotent stem cells, comprising the steps of:
- a. Attaching a population of pluripotent stem cells to a first volume of micro-carriers,b. Culturing the pluripotent stem cells on the first volume of micro-carriers,c. Removing the pluripotent stem cells from the first volume of micro-carriers, andd. Attaching the population of pluripotent stem cells to a second volume of micro-carriers.
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Abstract
The present invention is directed to methods for the growth, expansion and differentiation of pluripotent stem cells on micro-carriers.
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Citations
10 Claims
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1. A method for the propagation of pluripotent stem cells, comprising the steps of:
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a. Attaching a population of pluripotent stem cells to a first volume of micro-carriers, b. Culturing the pluripotent stem cells on the first volume of micro-carriers, c. Removing the pluripotent stem cells from the first volume of micro-carriers, and d. Attaching the population of pluripotent stem cells to a second volume of micro-carriers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method to differentiate pluripotent stem cells to insulin-expressing cells on micro-carriers comprising the steps of:
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a. Attaching the pluripotent stem cells to a volume of micro-carriers, b. Differentiating the pluripotent stem cells to cells expressing markers characteristic of the definitive endoderm lineage, c. Differentiating the cells expressing markers characteristic of the definitive endoderm lineage into cells expressing markers characteristic of the pancreatic endoderm lineage, d. Differentiating the cells expressing markers characteristic of the pancreatic endoderm lineage into cells expressing markers characteristic of the pancreatic endocrine lineage, and e. Differentiating the cells expressing markers characteristic of the pancreatic endocrine lineage into insulin-expressing cells.
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Specification