METHODS AND SYSTEMS FOR NUCLEIC ACID SEQUENCING VALIDATION, CALIBRATION AND NORMALIZATION
First Claim
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1. A system for performing quality control for nucleic acid sample sequencing, the system comprising:
- a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences,wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto,wherein the nucleic acid sequences of each group differ from each other, andwherein the nucleic acid sequences are synthetically derived.
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Abstract
A system for performing quality control for nucleic acid sample sequencing is disclosed. The system has a set of solid supports, each support having attached thereto a plurality of nucleic acid sequences. The set has plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived. A method of preparing a quality control for performing nucleic acid sample sequencing and a method of validating a nucleic acid sequencing instrument are also disclosed.
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26 Claims
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1. A system for performing quality control for nucleic acid sample sequencing, the system comprising:
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a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto, wherein the nucleic acid sequences of each group differ from each other, and wherein the nucleic acid sequences are synthetically derived. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method of preparing a quality control for performing nucleic acid sample sequencing, comprising:
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generating a plurality of synthetic nucleic acid sequences, wherein each synthetic nucleic acid sequence differs from another nucleic acid sequence; attaching each of the synthetic nucleic acid sequences to solid supports in plural groups of solid supports, wherein the solid supports in each group have the same synthetic nucleic acid sequence attached thereto; and combining each group of solid supports with the synthetic nucleic acid sequences attached to create a control set of solid supports for performing nucleic acid sample sequencing. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A method of performing nucleic acid sequencing validation, comprising:
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placing a set of solid supports each having a plurality of synthetic nucleic acid sequences attached thereto in a detection area of a nucleic acid sequencing instrument, wherein the set of solid supports comprises plural of groups of solid supports each of the solid supports in a group having the same synthetic nucleic acid sequences attached thereto and the solid supports in differing groups having differing synthetic nucleic acid sequences attached thereto; generating a focal map to identify the location of each solid support relative to the detection area of the nucleic acid sequencing instrument; performing one or more ligation cycles to attach a dye-labeled probe sequence to the nucleic acid sequences attached to the solid supports; detecting the dye-labeled probes attached to each of the nucleic acid sequence; measuring the intensities of the dye-labeled probes; and comparing the measured intensities to a threshold value to determine if the instrument is functioning validly.
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Specification