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PYROPHOSPHOROLYSIS ACTIVATED POLYMERIZATION (PAP)

  • US 20100129871A1
  • Filed: 11/04/2009
  • Published: 05/27/2010
  • Est. Priority Date: 02/23/2000
  • Status: Active Grant
First Claim
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1. A process which comprises serial coupling of two reactions, the first reaction being activation of an inactive oligonucleotide which, if not activated, would prevent the oligonucleotide from being extended on a nucleic acid template and the second reaction being an extension of the activated oligonucleotide on the nucleic acid template, wherein the inactive oligonucleotide has a 3′

  • match with the nucleic acid template and wherein the inactive oligonucleotide is activated by a nucleic acid metabolizing enzyme.

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