MAPPING OF GENOMIC INTERACTIONS
First Claim
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1. A method, comprising:
- a) contacting a genomic interaction library with a plurality of unique primers under conditions such that ligation mediated amplification generates a second genomic interaction library; and
b) amplifying said second genomic library with a single pair of PCR primers, wherein said single pair of PCR primers amplify all members of said second genomic library.
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Abstract
The present invention relates to genomic analysis. In particular, the present invention provides methods and compositions for mapping genomic interactions.
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Citations
20 Claims
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1. A method, comprising:
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a) contacting a genomic interaction library with a plurality of unique primers under conditions such that ligation mediated amplification generates a second genomic interaction library; and b) amplifying said second genomic library with a single pair of PCR primers, wherein said single pair of PCR primers amplify all members of said second genomic library. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method, comprising;
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a) contacting nucleic acid with a cross-linking agent under conditions such that interacting chromatin segments are cross-linked; b) digesting said cross-linked chromatin segments to generate digested chromatin segments; c) ligating said digested chromatin segments to generate a genomic interaction library; d) contacting said genomic interaction library with a plurality of unique primers under conditions such that ligation mediated amplification generates a second genomic interaction library, and e) amplifying said second genomic library with a single pair of PCR primers, wherein said single pair of PCR primers amplify all members of said second genomic library.
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Specification