CROSS-LINKED ENZYME MATRIX AND USES THEREOF
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Accused Products
Abstract
An electrochemical sensor system and membrane and method thereof for increased accuracy and effective life of electrochemical and enzyme sensors.
35 Citations
49 Claims
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1-22. -22. (canceled)
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23. A composite membrane for an enzyme sensor, comprising:
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an inner membrane layer comprising a restorable polymerizable membrane, the inner membrane layer adapted to function as an interference rejection membrane; an outer membrane layer comprising polyurethane, and adapted to be disposed adjacent a flow channel during use; and an enzyme layer disposed between and in contact with the inner and the outer membrane layers, said enzyme layer comprising a matrix comprising; at least one enzyme selected from the group consisting of creatinase and sarcosine oxidase; a cross-linking agent; and an enzyme stabilizer selected from the group consisting of polyethyleneimine, polypropyleneimine, poly(N-vinylimidazole), polyallylamine, polyvinylpyridine, polyvinylpyrrolidone, polylysine, and protamine; wherein the outer membrane is adapted to control diffusion of an analyte into the enzyme layer. - View Dependent Claims (24, 26, 27, 28, 29, 30, 32, 34, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48)
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25. (canceled)
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31. (canceled)
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33. (canceled)
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35-38. -38. (canceled)
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49. A composite membrane for an enzyme sensor, comprising:
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an inner membrane layer comprising a restorable polymerizable membrane, the inner membrane layer adapted to function as an interference rejection membrane; an outer membrane layer comprising polyurethane, and adapted to be disposed adjacent a flow channel during use; and an enzyme layer disposed between and in contact with the inner and the outer membrane layers, said enzyme layer comprising a matrix comprising; creatininase; creatinase; sarcosine oxidase; glutaraldehyde; and polyethyleneimine, wherein the outer membrane is adapted to control diffusion of an analyte into the enzyme layer.
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Specification