METHODS FOR ELECTROCHEMICAL DETECTION/QUANTIFICATION OF A NUCLEIC ACID

• US 20100133117A1
• Filed: 08/08/2006
• Published: 06/03/2010
• Est. Priority Date: 08/08/2006
• Status: Abandoned Application

• Current Assignee: Agency For Science Technology and Research
• Original Assignee: Agency For Science Technology and Research
• Inventors: Gao, Zhiqiang
• Application Number:
  US12/376,782
• Publication Number:
  US 20100133117A1
• Time in Patent Office: Days
• US Class Current: 205/777.500
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2525/101 incorporating non-naturally occurring nucleotides, e.g. inosine
• CPC Class Codes: C12Q 2525/107 incorporating a peptide nucleic acid
• CPC Class Codes: C12Q 2533/101 Primer extension
• CPC Class Codes: G01N 27/447 using electrophoresis

Abstract

The invention provides methods and kits for the electrochemical detection and/or quantification of a target nucleic acid molecule by means of a detection electrode. In one method there is immobilized on the detection electrode a peptide nucleic acid (PNA) capture molecule, which has a nucleotide sequence that is at least partially complementary to at least a portion of the target nucleic acid molecule. The electrode is contacted with a solution expected to include the target nucleic acid molecule and the target nucleic acid molecule allowed to hybridize to the PNA, thereby allowing the formation of a complex between the PNA capture molecule and the target nucleic acid molecule. A polymerisable positively chargeable precursor is added, which associates to the complex formed between the PNA capture molecule and the target nucleic acid molecule. A suitable reactant molecule is added, initiating the polymerisation of the polymerisable positively chargeable precursor and the formation of an electroconductive polymer. An electrochemical measurement at the detection electrode is performed. In another method a nucleic acid capture molecule is immobilized on the electrode. In this method a polymerisable positively chargeable precursor, a suitable substrate molecule, and an enzyme attached to a detection probe nucleic acid molecule are used. The detection probe nucleic acid molecule is at least partially complementary to at least a portion of the target nucleic acid molecule. The detection probe hybridizes to a portion of the target nucleic acid that is different from the portion to which the capture nucleic acid molecule hybridises.

Claims

1. A method of electrochemically detecting and/or quantifying a target nucleic acid molecule by means of a detection electrode, the method comprising:

(a) providing a detection electrode;

(b) immobilizing on said detection electrode a peptide nucleic acid (PNA) capture molecule, which has a nucleotide sequence that is at least partially complementary to at least a portion of the target nucleic acid molecule;

(c) contacting the electrode with a solution expected to comprise the target nucleic acid molecule;

(d) allowing the target nucleic acid molecule to hybridize to the PNA capture molecule on the electrode, thereby allowing the formation of a complex between said PNA capture molecule and said target nucleic acid molecule;

(e) adding a polymerisable positively chargeable precursor, wherein said polymerisable positively chargeable precursor has an electrostatic net charge that is complementary to the electrostatic net charge of the target nucleic acid molecule, such that (i) said polymerisable positively chargeable precursor associates to the complex formed between said PNA capture molecule and said target nucleic acid molecule, and (ii) the polymerisation of said precursor can be carried out by means of a suitable reactant molecule;

(f) adding a suitable reactant molecule, thereby initiating the polymerisation of said polymerisable positively chargeable precursor, wherein an electroconductive polymer is formed from the polymerisable precursor and this electroconductive polymer is associated with the complex formed between the PNA capture molecule and the target nucleic acid molecule;

(g) performing an electrochemical measurement at the detection electrode; and

(h) detecting and/or quantifying the presence of the target nucleic acid molecule based on the electrochemical measurement.

3. The method of claim 1, wherein the reactant molecule is an oxidant.

4. The method of claim 3, wherein the oxidant is at least one of a ruthenium tris(bipyridinium) complex, a persulfate, a peroxide, a chromate, a dichromate, a manganate, a permanganate, oxygen, ozone, a persulfate, a halogen, a chlorite, a chloride, a perchloride, a chlorate, a iodate, a nitrate, a sulfoxide and osmium tetroxide.

5. The method of claim 1, wherein (f) comprises exposing the reactant molecule to a suitable initiator.

6. The method of claim 5, wherein the initiator is one of a halogen molecule, an azo compound, a persulfate molecule, and a peroxide compound.

8. The method of claim 1, wherein (f) comprises exposing the reactant molecule to a suitable catalyst.

9. The method of claim 8, wherein the catalyst is selected from the group consisting of a metal chloride, a metal bromide, a metal sulphate and an enzyme.

10. The method of claim 9, wherein the reactant molecule is a substrate molecule for said catalyst.

11. The method of claim 9, wherein said catalyst is in solution or is coupled to a detection probe nucleic acid molecule, wherein said detection probe nucleic acid molecule is complementary to at least a portion of the target nucleic acid molecule.

12. The method of claim 11, wherein said detection probe nucleic acid molecule has a nucleic acid sequence of a length of about 5 to about 50 bp.

13. The method of claim 9, wherein said catalyst is an enzyme.

14. The method of claim 13, wherein said enzyme is a peroxidase enzyme or an oxidase enzyme.

15. The method of claim 1, wherein said PNA capture molecule has a nucleic acid sequence of a length of about 7 to about 30 bp.

26. The method of claim 1, wherein said target nucleic acid molecule is DNA or RNA.

27. The method of claim 1, wherein said target nucleic acid molecule comprises a pre-defined sequence.

28. The method of claim 1, wherein the target nucleic acid molecule comprises at least one single-stranded region.

29. The method of claim 28, wherein said predefined sequence is a single-stranded region.

30. The method of claim 14, wherein said peroxidase enzyme is a haem peroxidase.

31. The method of claim 30, wherein said haem peroxidase is selected from the group consisting of horseradish peroxidase, cytochrome c peroxidase, glutathione peroxidase, myeloperoxidase, thyroid peroxidase, eosinophil peroxidase, lactoperoxidase, ascorbate peroxidase, peroxidasin, prostaglandin H synthase, E. coli catalase-peroxidase, M. tuberculosis catalase-peroxidase, Bacteroides fragilis catalase-peroxidase, lignin peroxidase, plant ascorbate peroxidase, Haem chloroperoxidase, manganese peroxidase, stigma specific peroxidase, Euphorbia characias latex peroxidase, Arthromyces ramosus peroxidase, sorghum grain peroxidase SPC4, soybean peroxidase, Phanerochaete chrysosporium manganese-dependent peroxidase, and lacrimal gland peroxidase.

32. The method of claim 14, wherein said oxidase enzyme is laccase.

34. The method of claim 1, wherein the positively chargeable precursor is an aromatic amine.

35. The method of claim 34, wherein said aromatic amine is selected from the group consisting of aniline, pyridineamine, pyrrole and imidazole.

36. The method of claim 34, wherein the target nucleic acid molecule is comprised in a solution of a pH selected in the range of about 1.7 to about 7.0, when said polymerisable positively chargeable precursor is added.

37. The method of claim 34, wherein the pH is brought to a value in the range of about 1.7 to about 7.0 after said polymerisable positively chargeable precursor has been added.

40. The method of claim 1, wherein the target nucleic acid molecule is comprised in a sample selected from the group consisting of a soil sample, an air sample, an environmental sample, a cell culture sample, a bone marrow sample, a rainfall sample, a fallout sample, a space sample, an extraterrestrial sample, a sewage sample, a ground water sample, an abrasion sample, an archaeological sample, a food sample, a blood sample, a serum sample, a plasma sample, a urine sample, a stool sample, a semen sample, a lymphatic fluid sample, a cerebrospinal fluid sample, a naspharyngeal wash sample, a sputum sample, a mouth swab sample, a throat swab sample, a nasal swab sample, a bronchoalveolar lavage sample, a bronchial secretion sample, a milk sample, an amniotic fluid sample, a biopsy sample, a nail sample, a hair sample, a skin sample, a cancer sample, a tumour sample, a tissue sample, a cell sample, a cell lysate sample, a virus culture sample, a forensic sample, an infection sample, a nosocomial infection sample, a production sample, a drug preparation sample, a biological molecule production sample, a protein preparation sample, a lipid preparation sample, a carbohydrate preparation sample, a solution of a nucleotide, a solution of polynucleotide, a solution of a nucleic acid, a solution of a peptide, a solution of a polypeptide, a solution of an amino acid, a solution of a protein, a solution of a synthetic polymer, a solution of a biochemical composition, a solution of an organic chemical composition, a solution of an inorganic chemical composition, a solution of a lipid, a solution of a carbohydrate, a solution of a combinatory chemistry product, a solution of a drug candidate molecule, a solution of a drug molecule, a solution of a drug metabolite, a suspension of a cell, a suspension of a virus, a suspension of a microorganism, a suspension of a metal, a suspension of metal alloy, a solution of a metal ion, and any combination thereof.

2. (canceled)

7. (canceled)

16. (canceled)

17. A method of electrochemically detecting and/or quantifying a target nucleic acid molecule by means of a detection electrode, the method comprising:

(a) providing a detection electrode;

(b) immobilizing on said detection electrode a nucleic acid capture molecule, which has a nucleotide sequence that is at least partially complementary to at least a portion of the target nucleic acid molecule;

(c) contacting the electrode with a solution expected to comprise the target nucleic acid molecule;

(d) allowing the target nucleic acid molecule to hybridize to the nucleic acid capture molecule on the electrode, thereby allowing formation of a complex between said nucleic acid capture molecule and said target nucleic acid molecule;

(e) adding a polymerisable positively chargeable precursor, wherein said polymerisable positively chargeable precursor has an electrostatic net charge that is complementary to the electrostatic net charge of the target nucleic acid molecule, such that (i) said polymerisable positively chargeable precursor associates with the complex formed between said nucleic acid capture molecule and said target nucleic acid molecule, and (ii) the polymerisation of said precursor can be carried out by means of a suitable enzyme and a substrate molecule;

(f) adding a suitable substrate molecule;

(g) adding an enzyme attached to a detection probe nucleic acid molecule, wherein said probe nucleic acid molecule is at least partially complementary to at least a portion of the target nucleic acid molecule, wherein the detection probe hybridizes to a portion of the target nucleic acid different from the portion to which the capture nucleic acid molecule hybridizes, thereby (i) allowing the detection probe nucleic acid molecule to hybridise to the target nucleic acid molecule, and (ii) catalysing the polymerisation of said polymerisable positively chargeable precursor, wherein an electro conductive polymer is formed from the polymerisable precursor and this electroconductive polymer is associated with the complex formed between the nucleic acid capture molecule and the target nucleic acid molecule;

(h) performing an electrochemical measurement at the detection electrode, and;

(i) detecting and/or quantifying the presence of the target nucleic acid molecule.

19. The method of claim 17, wherein the substrate molecule is an oxidant.

20. The method of claim 19, wherein the oxidant is at least one of a persulfate, a peroxide, a chromate, a dichromate, a manganate, a permanganate, oxygen, ozone, a halogene, a chlorite, a chloride, a perchloride, a chlorate, a iodate, a nitrate, a sulfoxide and osmium tetroxide.

21. The method of claim 17, wherein said enzyme is a peroxidase enzyme or an oxidase enzyme.

22. The method of claim 17, wherein said detection probe nucleic acid molecule has a nucleic acid sequence of a length of about 5 to about 50 bp.

23. The method of claim 17, wherein said nucleic acid capture molecule has a nucleic acid sequence of a length of about 7 to about 30 bp.

25. The method of claim 17, wherein said nucleic acid capture molecule is one of DNA, RNA and PNA.

18. (canceled)

24. (canceled)

33. (canceled)

38. (canceled)

39. (canceled)

41. A kit for the electrochemical detection of a target nucleic acid molecule, said kit comprising

(a) a detection electrode,

(b) a PNA capture molecule, which has a nucleotide sequence that is at least partially complementary to at least a portion of the target nucleic acid molecule,

(c) a polymerisable positively chargeable precursor, wherein the electrostatic net charge of said polymerisable positively chargeable precursor is complementary to the electrostatic net charge of the target nucleic acid molecule, and

(d) a suitable reactant molecule.

42-53. -53. (canceled)

54. A kit for the electrochemical detection of a target nucleic acid molecule, said kit comprising

(a) a detection electrode,

(b) a nucleic acid capture molecule, which has a nucleotide sequence that is at least partially complementary to at least a portion of the target nucleic acid molecule,

(c) a polymerisable positively chargeable precursor, wherein the electrostatic net charge of said polymerisable positively chargeable precursor is complementary to the electrostatic net charge of the target nucleic acid molecule,

(d) a substrate molecule, and

(e) an enzyme attached to a probe nucleic acid molecule, wherein said probe nucleic acid molecule is at least partially complementary to at least a portion of the target nucleic acid molecule.

55-66. -66. (canceled)