Human Interferon Molecules and Their Uses
First Claim
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1. A hIFN polypeptide comprising one or more non-naturally encoded amino acids having at least one modulated biological activity.
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Abstract
Modified human interferon polypeptides and uses thereof are provided.
109 Citations
103 Claims
- 1. A hIFN polypeptide comprising one or more non-naturally encoded amino acids having at least one modulated biological activity.
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54. An isolated nucleic acid comprising a polynucleotide that hybridizes under stringent conditions to SEQ ID NO:
- 21 or SEQ ID NO;
22, wherein the polynucleotide comprises at least one selector codon. - View Dependent Claims (55, 69, 70)
- 21 or SEQ ID NO;
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71. A method of making a hIFN polypeptide comprising a non-naturally encoded amino acid, the method comprising, culturing cells comprising a polynucleotide or polynucleotides encoding a hIFN polypeptide and comprising a selector codon, an orthogonal RNA synthetase and an orthogonal tRNA under conditions to permit expression of the hIFN polypeptide comprising a non-naturally encoded amino acid;
- and purifying the hIFN polypeptide.
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72. A method of increasing serum half-life or circulation time of a hIFN polypeptide, the method comprising substituting one or more non-naturally encoded amino acids for any one or more naturally occurring amino acids in the hIFN polypeptide.
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75. A hIFN polypeptide comprising a non-naturally encoded amino acid , wherein the non-naturally encoded amino acid is substituted at a position selected from the group consisting of 23, 24, 25, 26, 27, 28, 30, 31, 32, 33, 128, 129, 131, 132, 133, 134, 135, 136, 137, 158, 159, 160, 161, 162, 163, 164, 165 of SEQ ID NO:
- 2 or the corresponding amino acids in SEQ ID NO;
1 or 3. - View Dependent Claims (76, 77, 78, 79, 80, 81)
- 2 or the corresponding amino acids in SEQ ID NO;
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82. A hIFN polypeptide comprising one or more amino acid substitution, addition or deletion that increases the expression of the hIFN polypeptide in a recombinant host cell.
- 83. A hIFN polypeptide comprising a water soluble polymer linked by a covalent bond to the hIFN polypeptide at a single amino acid.
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95. A method of modulating immunogenicity of a hIFN polypeptide, the method comprising substituting one or more non-naturally encoded amino acids for any one or more naturally occurring amino acids in the hIFN polypeptide.
Specification