DETECTING HEPATITIS B VIRUS
First Claim
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1. A method for detecting hepatitis B in a test sample, comprising:
- (a) providing a test sample; and
(b) contacting said test sample with a first antibody for a time and under conditions sufficient to form middle hepatitis B surface protein (M-HBsAg)/first antibody complexes, presence of said complexes indicating detection of hepatitis B present said sample.
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Abstract
This invention is related to methods for detecting hepatitis B virus by determining the level of hepatitis B virus surface antigen protein. This invention is also related to methods for detecting mutant hepatitis B virus surface antigen protein and kits for detecting hepatitis B virus.
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Citations
17 Claims
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1. A method for detecting hepatitis B in a test sample, comprising:
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(a) providing a test sample; and (b) contacting said test sample with a first antibody for a time and under conditions sufficient to form middle hepatitis B surface protein (M-HBsAg)/first antibody complexes, presence of said complexes indicating detection of hepatitis B present said sample. - View Dependent Claims (2, 3, 4, 5)
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6. A method for detecting a mutant S-HBsAg in a test sample comprising:
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(a) providing a test sample; (b) contacting said test sample with a first antibody for a time and under conditions sufficient to form middle hepatitis B surface protein (M-HBsAg)/first antibody complexes; (c) contacting said test sample with a second antibody for a time and under conditions sufficient to form small hepatitis B surface protein (S-HBsAg)/second antibody complexes; and (d) comparing level of M-HBsAg/first antibody complexes to level of S-HBsAg/second antibody complexes, wherein a reduced level of S-HBsAg/second antibody complexes to M-HBsAg/first antibody complexes indicates detection of a mutant S-HBsAg in the test sample. - View Dependent Claims (7, 8, 9)
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10. A method for detecting a mutant S-HBsAg in a test sample, comprising:
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(a) providing a test sample; (b) contacting said test sample with a first antibody for a time and under conditions sufficient to form small hepatitis B surface protein (S-HBsAg)/antibody complex wherein a reduced level of S-HBsAg/antibody complex in comparison to a control indicates detection of a mutant S-HBsAg in the test sample. - View Dependent Claims (11, 12)
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13. A kit for detecting hepatitis B comprising:
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(a) at least one antibody that binds to M-HBsAg; and (b) at least one antibody that binds to S-HBsAg. - View Dependent Claims (14, 15, 16, 17)
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Specification