SUPPORTED REAGENTS, METHODS, AND DEVICES

• US 20100136554A1
• Filed: 04/25/2008
• Published: 06/03/2010
• Est. Priority Date: 04/25/2007
• Status: Active Grant

• Current Assignee: Diasorin Italia SPA
• Original Assignee: 3M Innovative Properties Company (3M Company)
• Inventors: Bedingham, William, Faletti, John C., Danielson, Michael E., Parthasarathy, Ranjani V.
• Granted Patent: US 8,835,157 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

Methods of providing at least one reagent for use in a device for processing sample material, delivering at least one reagent to a device for processing sample material, and adding at least one reagent to at least one of the steps in a process for detecting or assaying a nucleic acid; a support film coated with a dry reagent layer; and a device for processing sample material having a support film coated with a dry reagent layer contained within at least one chamber of the device are disclosed.

Claims

1. A method of providing at least one reagent for processing sample material, the method comprising:

providing a support film coated with a reagent layer which includes the at least one reagent; and

dimensioning the reagent layer or the reagent layer and support film to fit within at least one chamber of a device for processing sample material, wherein the at least one chamber can contain or channel a fluid;

wherein the dimensions of the reagent layer or the reagent layer and support film allow space between walls of the chamber and edges and major surfaces of the dimensioned reagent layer or the reagent layer and support film.

4. The method of claim 1 wherein the at least one reagent can be used in at least one of a step of sample preparation, a step of nucleic acid amplification, and a step of detection in a process for detecting or assaying a nucleic acid.

6. The method of claim 1, wherein the reagent layer further includes a matrix material selected from the group consisting of a water soluble polymer, a carbohydrate, and a combination thereof.

8. The method of claim 1, wherein the support film coated with the reagent layer is further dimensioned to provide a pre-determined amount of the at least one reagent.

9. The method of claim 1, wherein the device for processing sample material is a microfluidic device.

2. A method of delivering at least one reagent to a device for processing sample material, the method comprising:

providing a support film coated with a reagent layer which includes the at least one reagent;

placing the support film coated with the reagent layer or the reagent layer portion of the support film coated with the reagent layer within at least one chamber of a device for processing sample material, wherein the at least one chamber can contain or channel a fluid; and

wherein the reagent layer or the reagent layer and support film are dimensioned to fit within the at least one chamber of the device for processing sample material;

wherein the dimensions of the reagent layer or the reagent layer and support film allow space between walls of the chamber and edges and major surfaces of the dimensioned reagent layer or the reagent layer and support film.

27. The method of claim 2 wherein the at least one reagent can be used in at least one of a step of sample preparation, a step of nucleic acid amplification, and a step of detection in a process for detecting or assaying a nucleic acid.

28. The method of claim 2, wherein the reagent layer further includes a matrix material selected from the group consisting of a water soluble polymer, a carbohydrate, and a combination thereof.

29. The method of claim 2, wherein the support film coated with the reagent layer is further dimensioned to provide a pre-determined amount of the at least one reagent.

30. The method of claim 2, wherein the device for processing sample material is a microfluidic device.

3. (canceled)

5. (canceled)

7. (canceled)

10. (canceled)

11. A method of adding at least one reagent to at least one step in a process for detecting or assaying a nucleic acid, the method comprising:

providing a support film coated with a dry reagent layer which includes the at least one reagent;

placing the support film coated with the dry reagent layer or the dry reagent layer portion of the support film coated with the dry reagent layer in at least one chamber which can contain or channel a fluid, wherein the at least one step is conducted in the at least one chamber, and wherein the at least one step is selected from the group consisting of sample preparation, nucleic acid amplification, and detection; and

contacting the dry reagent layer with a fluid which dissolves, disperses, or suspends the at least one reagent in the reagent layer.

14. The method of claim 11, wherein the dry reagent layer further includes a matrix material selected from the group consisting of a water soluble polymer, a carbohydrate, and a combination thereof.

17. The method of claim 11, wherein the reagent layer portion of the support film coated with the reagent layer is dimensioned to fit within and is placed within the at least one chamber of the device for processing sample material.

12-13. -13. (canceled)

15-16. -16. (canceled)

18. (canceled)

19. A support film coated with a dry reagent layer, wherein the reagent layer or the reagent layer and support film are dimensioned to fit within at least one chamber of a microfluidic device.

22. The support film coated with the dry reagent layer of claim 19, wherein the dry reagent layer further includes a matrix material selected from the group consisting of a water soluble polymer, a carbohydrate, and a combination thereof.

20. A support film coated with a dry reagent layer, wherein the dry reagent layer comprises at least one reagent which can be used in at least one of a step of sample preparation, a step of nucleic acid amplification, and a step of detection in a process for detecting or assaying a nucleic acid.

31. The support film coated with the dry reagent layer of claim 20, wherein the dry reagent layer further includes a matrix material selected from the group consisting of a water soluble polymer, a carbohydrate, and a combination thereof.

21. (canceled)

23. A device for processing sample material, the device having a plurality of chambers which can contain or channel a fluid, wherein a support film coated with a dry reagent layer or the dry reagent layer portion of the support film coated with the dry reagent layer is dimensioned to fit within and is contained within at least one chamber of the device.

24. The device of claim 23, wherein the dry reagent layer includes at least one reagent which can be used in at least one of a step of sample preparation, a step of nucleic acid amplification, and a step of detection in a process for detecting or assaying a nucleic acid.

26. The device of claim 23, wherein the dry reagent layer further includes a matrix material selected from the group consisting of a water soluble polymer, a carbohydrate, and a combination thereof.

25. (canceled)