SUPPORTED REAGENTS, METHODS, AND DEVICES
First Claim
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1. A method of providing at least one reagent for processing sample material, the method comprising:
- providing a support film coated with a reagent layer which includes the at least one reagent; and
dimensioning the reagent layer or the reagent layer and support film to fit within at least one chamber of a device for processing sample material, wherein the at least one chamber can contain or channel a fluid;
wherein the dimensions of the reagent layer or the reagent layer and support film allow space between walls of the chamber and edges and major surfaces of the dimensioned reagent layer or the reagent layer and support film.
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Abstract
Methods of providing at least one reagent for use in a device for processing sample material, delivering at least one reagent to a device for processing sample material, and adding at least one reagent to at least one of the steps in a process for detecting or assaying a nucleic acid; a support film coated with a dry reagent layer; and a device for processing sample material having a support film coated with a dry reagent layer contained within at least one chamber of the device are disclosed.
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Citations
31 Claims
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1. A method of providing at least one reagent for processing sample material, the method comprising:
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providing a support film coated with a reagent layer which includes the at least one reagent; and dimensioning the reagent layer or the reagent layer and support film to fit within at least one chamber of a device for processing sample material, wherein the at least one chamber can contain or channel a fluid;
wherein the dimensions of the reagent layer or the reagent layer and support film allow space between walls of the chamber and edges and major surfaces of the dimensioned reagent layer or the reagent layer and support film. - View Dependent Claims (4, 6, 8, 9)
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2. A method of delivering at least one reagent to a device for processing sample material, the method comprising:
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providing a support film coated with a reagent layer which includes the at least one reagent; placing the support film coated with the reagent layer or the reagent layer portion of the support film coated with the reagent layer within at least one chamber of a device for processing sample material, wherein the at least one chamber can contain or channel a fluid; and
wherein the reagent layer or the reagent layer and support film are dimensioned to fit within the at least one chamber of the device for processing sample material;
wherein the dimensions of the reagent layer or the reagent layer and support film allow space between walls of the chamber and edges and major surfaces of the dimensioned reagent layer or the reagent layer and support film. - View Dependent Claims (27, 28, 29, 30)
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3. (canceled)
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5. (canceled)
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7. (canceled)
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10. (canceled)
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11. A method of adding at least one reagent to at least one step in a process for detecting or assaying a nucleic acid, the method comprising:
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providing a support film coated with a dry reagent layer which includes the at least one reagent; placing the support film coated with the dry reagent layer or the dry reagent layer portion of the support film coated with the dry reagent layer in at least one chamber which can contain or channel a fluid, wherein the at least one step is conducted in the at least one chamber, and wherein the at least one step is selected from the group consisting of sample preparation, nucleic acid amplification, and detection; and contacting the dry reagent layer with a fluid which dissolves, disperses, or suspends the at least one reagent in the reagent layer. - View Dependent Claims (14, 17)
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12-13. -13. (canceled)
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15-16. -16. (canceled)
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18. (canceled)
- 19. A support film coated with a dry reagent layer, wherein the reagent layer or the reagent layer and support film are dimensioned to fit within at least one chamber of a microfluidic device.
- 20. A support film coated with a dry reagent layer, wherein the dry reagent layer comprises at least one reagent which can be used in at least one of a step of sample preparation, a step of nucleic acid amplification, and a step of detection in a process for detecting or assaying a nucleic acid.
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21. (canceled)
- 23. A device for processing sample material, the device having a plurality of chambers which can contain or channel a fluid, wherein a support film coated with a dry reagent layer or the dry reagent layer portion of the support film coated with the dry reagent layer is dimensioned to fit within and is contained within at least one chamber of the device.
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25. (canceled)
Specification