SYSTEMS AND METHODS FOR PRODUCING BIOFUELS AND RELATED MATERIALS
First Claim
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1. A method of making a fuel from biomass material, the method comprising:
- providing a biomass material comprising a high molecular weight carbohydrate;
hydrolyzing the biomass material to provide a hydrolyzed biomass material;
combining the hydrolyzed biomass material with Clostridium phytofermentans cells in a medium, wherein a concentration of carbohydrates in the medium is greater than 20 mM; and
fermenting the hydrolyzed biomass material under conditions and for a time sufficient to produce a fuel.
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Abstract
Clostridium phytofermentans cells (American Type Culture Collection 700394T) and all other strains of the species can ferment materials such as biomass into useful products and coproducts, such as ethanol, hydrogen and organic acids. Compositions that include Clostridium phytofermentans are also disclosed.
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Citations
39 Claims
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1. A method of making a fuel from biomass material, the method comprising:
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providing a biomass material comprising a high molecular weight carbohydrate; hydrolyzing the biomass material to provide a hydrolyzed biomass material; combining the hydrolyzed biomass material with Clostridium phytofermentans cells in a medium, wherein a concentration of carbohydrates in the medium is greater than 20 mM; and fermenting the hydrolyzed biomass material under conditions and for a time sufficient to produce a fuel. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A fuel plant comprising:
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a hydrolysis unit configured to hydrolyze a biomass material comprising a high molecular weight carbohydrate; and a fermentor configured to house a medium and comprising Clostridium phytofermentans cells dispersed therein. - View Dependent Claims (9, 10, 11, 12)
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13. A method of making a fuel, the method comprising:
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combining Clostridium phytofermentans cells and a lignocellulosic material in a medium; and fermenting the lignocellulosic material under conditions and for a time sufficient to produce a fuel. - View Dependent Claims (14, 15, 16, 17, 18)
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19. A method of making a fuel, the method comprising:
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combining Clostridium phytofermentans cells and a material comprising a carbohydrate in a medium, wherein a concentration of the carbohydrate in the medium is greater than 40 mM; and fermenting the material comprising the carbohydrate under conditions and for a time sufficient to produce a fuel. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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35. A method comprising:
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combining Clostridium phytofermentans cells, a second microbe and a material comprising a carbohydrate in a medium; and fermenting the material comprising the carbohydrate. - View Dependent Claims (36, 37, 38)
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39. A composition comprising Clostridium phytofermentans and a second microbe.
Specification