METHOD OF DIFFERENTIATING MAMMALIAN PROGENITOR CELLS INTO INSULIN PRODUCING PANCREATIC ISLET CELLS

• US 20100136688A1
• Filed: 05/21/2009
• Published: 06/03/2010
• Est. Priority Date: 05/22/2008
• Status: Active Grant

• Current Assignee: Vesta Therapeutics, Inc.
• Original Assignee: Vesta Therapeutics, Inc.
• Inventors: HOYNOWSKI, Steven Michael, RUIZ, Joseph Charles
• Granted Patent: US 8,877,497 B2
• Time in Patent Office: Days
• US Class Current: 435/377
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: C12N 2500/30 Organic components
• CPC Class Codes: C12N 2500/36 Lipids
• CPC Class Codes: C12N 2500/90 Serum-free medium, which may still contain naturally-sourced components
• CPC Class Codes: C12N 2501/065 Modulators of histone acetylation
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 2506/14 from hepatocytes
• CPC Class Codes: C12N 2509/00 Methods for the dissociation of cells, e.g. specific use of enzymes
• CPC Class Codes: C12N 5/067 Hepatocytes
• CPC Class Codes: C12N 5/0676 Pancreatic cells
• CPC Class Codes: G01N 33/5014 for testing toxicity
• CPC Class Codes: G01N 33/5038 involving detection of metabolites per se
• CPC Class Codes: G01N 33/507 Pancreatic cells

Abstract

The invention relates to methods for differentiating progenitor cells into insulin producing pancreatic islet cells and compositions and methods for using such cells.

Claims

1. A method for differentiating progenitor cells into insulin-producing pancreatic cells, comprising:

(a) incubating the progenitor cells in serum-free pre-induction media comprising sodium butyrate; and

, subsequently,

(b) incubating the progenitor cell in induction media comprising knock-out replacement serum;

for a time sufficient to differentiate the progenitor cells into insulin producing pancreatic cells.

2. The method according to claim 1 in which the progenitor cells are human progenitor cells.

3. The method according to claim 1 in which the progenitor cells are hepatic stem cells.

4. The method according to claim 1 in which the progenitor cells are umbilical cord matrix stem cells.

5. The method according to claim 1 in which the progenitor cells are adipose stem cells.

6. The method according to claim 1 in which the induction media comprises about 2% knock-out replacement serum.

7. A method for differentiating progenitor cells into insulin-producing pancreatic cells, comprising:

(a) incubating the progenitor cells in serum-free pre-induction media comprising a histone deacetylase inhibitor (HDACI); and

, subsequently,

(b) incubating the progenitor cell in induction media comprising knock-out replacement serum;

for a time sufficient to differentiate the progenitor cells into insulin producing pancreatic cells.

8. The method according to claim 7 in which the HDACI is sodium butyrate, phenyl butyrate, retinoic acid, valproic acid, APHA Compound 8, Apicidin, ( ) Depudecin, Scriptaid, Sirtinol, and Trichostatin