UNIVERSAL BIOLOGICAL SAMPLE PROCESSING
First Claim
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1. A method for extracting nucleic acid fragments comprising the steps of:
- providing a biological sample;
suspending said biological sample in a suspension solution comprising a size stabilizer;
applying a mechanical force to said biological sample to extract nucleic acid molecules;
the application of said mechanical force in the presence of said size stabilizer results in said nucleic acid molecules being reduced to a median fragment size having a median number of base pairs, wherein at least 60% of said nucleic acid molecules have a number of base pairs that are within 50% of said median number of base pairs.
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Abstract
A method for preparing a sample by utilizing a mechanical force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range.
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Citations
20 Claims
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1. A method for extracting nucleic acid fragments comprising the steps of:
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providing a biological sample; suspending said biological sample in a suspension solution comprising a size stabilizer; applying a mechanical force to said biological sample to extract nucleic acid molecules; the application of said mechanical force in the presence of said size stabilizer results in said nucleic acid molecules being reduced to a median fragment size having a median number of base pairs, wherein at least 60% of said nucleic acid molecules have a number of base pairs that are within 50% of said median number of base pairs. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification