Droplet-Based Surface Modification and Washing
First Claim
1. A method of providing a bead-containing droplet with a reduced concentration of a substance, the method comprising:
- (a) providing a droplet microactuator comprising a droplet having a starting volume and comprising;
(i) one or more beads; and
(ii) a starting concentration and starting quantity of the substance;
(b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and
(c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising;
(i) a droplet comprising substantially all of the one or more beads and having a decreased concentration of the substance relative to the starting concentration; and
(ii) a droplet which is substantially lacking in the beads.
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Accused Products
Abstract
The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a bead-containing droplet with a reduced concentration of a substance is provided, the method including providing a droplet microactuator including a droplet having a starting volume and including one or more beads and a starting concentration and starting quantity of the substance. The method further includes conducting one or more droplet operations to merge a wash droplet with the droplet provided in the above step to yield a combined droplet and including one or more droplet operations to divide the combined droplet to yield a set of droplets including a droplet including substantially all of the one or more beads and having a decreased concentration of the substance relative to the starting concentration and a droplet which is substantially lacking in the beads.
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Citations
43 Claims
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1. A method of providing a bead-containing droplet with a reduced concentration of a substance, the method comprising:
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(a) providing a droplet microactuator comprising a droplet having a starting volume and comprising; (i) one or more beads; and (ii) a starting concentration and starting quantity of the substance; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising; (i) a droplet comprising substantially all of the one or more beads and having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is substantially lacking in the beads. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41)
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42. A method of providing a bead-containing droplet with a reduced concentration of a substance, the method comprising:
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(a) providing an electrowetting device comprising; (i) electrodes arranged to mediate droplet operations; (ii) a bead-containing droplet having a starting volume and comprising a starting concentration and starting quantity of the substance; (b) conducting droplet operations mediated by the electrodes to; (i) merge a wash droplet with the bead-containing droplet provided in step 1(a) to yield a combined droplet; and (ii) divide the combined droplet to yield a set of droplets comprising; (1) a droplet comprising substantially all of the one or more beads and having a decreased concentration of the substance relative to the starting concentration; and (2) a droplet which is substantially lacking in the beads. - View Dependent Claims (43)
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Specification