COMPOSITION FOR STIMULATING FORMATION OF VASCULAR STRUCTURES

• US 20100143476A1
• Filed: 02/14/2008
• Published: 06/10/2010
• Est. Priority Date: 02/14/2007
• Status: Abandoned Application

• Current Assignee: Indiana University Research & Technology Corporation (Indiana University)
• Original Assignee: Indiana University Research & Technology Corporation (Indiana University)
• Inventors: Johnstone, Brian, Traktuev, Dmity O., March, Keith L.
• Application Number:
  US12/526,656
• Publication Number:
  US 20100143476A1
• Time in Patent Office: Days
• US Class Current: 424/484
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 9/0024 Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue compositions for intravenous administration, normal injectable solutions or dispersions for, e.g. subcutaneous administration A61K9/0019; brain implants A61K9/0085; (coated) prostheses, catheters or stents A61L
• CPC Class Codes: C12N 2533/52 Fibronectin; Laminin
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 5/0667 Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
• CPC Class Codes: C12N 5/0692 Stem cells; Progenitor cells; Precursor cells

Abstract

Cell based compositions and methods are provided for inducing the formation of vascular structures in a warm blooded vertebrate. In one embodiment the composition comprises purified endothelial progenitor cells and adipose stromal cells and the method of stimulating the formation of vascular structures comprises the steps of implanting the composition in a host organism.

Claims

1. A composition comprising a mixture of purified endothelial cells and purified adipose stromal cells.

2. The composition of claim 1 wherein the endothelial cells are endothelial progenitor cells.

3. The composition of claim 2 wherein the endothelial progenitor cells are isolated from umbilical cord blood.

4. The composition of claim 1 wherein the composition further comprises an extracellular matrix protein or glycoprotein.

5. The composition of claim 1 further comprising a biocompatible polymer.

6. The composition of claim 5 wherein the biocompatible polymer is selected from the group consisting of collagen, peptides, polyglycol acid (PGA), polylactic acid (PLA) or a co-polymer of PGA and PLA.

7. The composition of claim 5 wherein the composition comprises collagen and fibronectin.

8. The composition of claim 1 wherein said mixture of cells is surrounded by a biocompatible matrix, comprising a biocompatible polymer selected from the group consisting of collagen, peptides, polyglycol acid (PGA), polylactic acid (PLA), and co-polymers of PGA and PLA.

9. The composition of claim 1 wherein said mixture of cells is surrounded by a hydrogel, alginate, collagen/fibronectin, PuraMatrix™

Peptide Hydrogel or MATRIGEL™

matrix.

10. The composition of claim 1 wherein said mixture of cells further comprises a pharmaceutically acceptable carrier, wherein the mixture of cells is suspended in said carrier.

11. The composition of claim 1 wherein said purified endothelial cells and purified adipose stromal cells are both native cell populations.

12. A method of creating a vessel network, comprising the steps of

mixing a purified population of endothelial cells with a purified population of adipose stromal cells to produce a mixture of cells;

incubating the mixture of cells under conditions conducive for growth of said cells, resulting in the formation of a network of vessels.

13. The method of claim 12 wherein said endothelial cells are endothelial progenitor cells.

14. The method of claim 13 wherein said endothelial cells are endothelial colony forming cells.

15. The method of claim 12 wherein the mixture of cells is surrounded by a biocompatible matrix.

16. The method of claim 12 wherein said incubating step comprises placing the mixture of cells into a warm blooded vertebrate.

17. The method of claim 16 wherein said purified endothelial cells and purified adipose stromal cells are both native autologous cell populations relative to said warm blooded vertebrate.

18. The method of claim 16 wherein the mixture of cells is injected into said vertebrate at the site where the formation of a network of vessels is desired.

19. The method of claims 18 wherein said mixture of cells further comprises a biocompatible polymer.

20. The method of claim 16 wherein the mixture of cells is surrounded by a biocompatible matrix and the cells are surgically implanted into said vertebrate.

21. A kit for inducing the formation of vascular networks, said kit comprising

a purified population of endothelial cells;

and

a purified population of adipose stromal cells.

22. The kit of claim 21 further comprising a biocompatible polymer.

23. The kit of claim 22 wherein said biocompatible polymer is selected from the group consisting of collagen, fibronectin, polyglycol acid (PGA), polylactic acid (PLA) or a co-polymer of PGA and PLA.

24. The kit of claim 21 wherein the endothelial cells are endothelial progenitor cells.

25. The kit of claim 21 further comprising a container comprising collagen and a container comprising fibronectin.

26. The kit of claim 21 further comprising FBS, and EGM-2.