METHOD AND COMPUTER PROGRAM PRODUCT FOR DISTINGUISHING AND SORTING SEEDS CONTAINING A GENETIC ELEMENT OF INTEREST
First Claim
1. A method for distinguishing seeds containing a genetic element of interest from a bulk sample, said method comprising:
- associating a fluorescent protein marker with at least some of the seeds containing a genetic element of interest;
placing a portion of the seeds from the bulk sample within an open-faced enclosure configured for use as a portable table-top evaluating device;
exciting at least some of the portion of seeds with a light source;
manually inspecting the excited seeds; and
manually sorting the excited seeds based on the presence or absence of the protein marker.
1 Assignment
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Accused Products
Abstract
A method for distinguishing and sorting seeds containing a genetic element of interest from a bulk sample. In various embodiments, the present invention comprises associating a marker with at least some of the seeds containing a genetic element of interest of the bulk sample, exciting the seeds using an photonic emitting device, evaluating at least some of the seeds of the bulk sample for the presence or absence of the marker, and sorting the seeds containing a genetic element of interest based on the presence or absence of the marker. In various other embodiments, the method may comprise associating a red fluorescent protein marker with at least some of the seeds containing a genetic element of interest of the bulk sample, evaluating at least some of the seeds of the bulk sample for the presence of the red fluorescent protein marker using an evaluating device, and sorting the seeds containing a genetic element of interest based on the presence of the red fluorescent protein marker. In some embodiments, the red fluorescent protein marker is discernable when excited by a certain energy and the evaluating step comprises exciting the seeds containing a genetic element of interest with the certain energy and detecting an emission resulting at least in part from the exciting step.
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Citations
24 Claims
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1. A method for distinguishing seeds containing a genetic element of interest from a bulk sample, said method comprising:
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associating a fluorescent protein marker with at least some of the seeds containing a genetic element of interest; placing a portion of the seeds from the bulk sample within an open-faced enclosure configured for use as a portable table-top evaluating device; exciting at least some of the portion of seeds with a light source; manually inspecting the excited seeds; and manually sorting the excited seeds based on the presence or absence of the protein marker. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for distinguishing seeds containing a genetic element of interest from a bulk sample, said method comprising:
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associating a red fluorescent protein marker with at least some of the seeds containing a genetic element of interest; loading a portion of the seeds from the bulk sample into a hopper and feeding the portion of seeds onto a tray defining a tray length; automatically moving the portion of seeds along the tray length; exciting at least some of the portion of seeds on the tray with an LED light source; manually inspecting the excited seeds; and manually sorting the seeds based on the presence or absence of the red fluorescent protein marker. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A method for distinguishing seeds containing a genetic element of interest from a bulk sample, the method comprising:
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associating a red fluorescent protein marker with at least some of the seeds containing a genetic element of interest; loading a portion of the seeds from the bulk sample into a hopper; exciting at least some of the portion of seeds with an LED light source; automatically feeding the portion of seeds past an image sensing device; and automatically sorting the excited seeds based on the presence or absence of the red fluorescent protein marker. - View Dependent Claims (22, 23, 24)
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Specification