Cells Modified or Altered for a Rice-diverged Glycosyltransferase

• US 20100143915A1
• Filed: 09/01/2009
• Published: 06/10/2010
• Est. Priority Date: 09/09/2008
• Status: Abandoned Application

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Cao, Peijian, Bartley, Laura E., Ronald, Pamela C., Jung, Ki-Hong
• Application Number:
  US12/552,155
• Publication Number:
  US 20100143915A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12N 15/8245 involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
• CPC Class Codes: C12N 9/1048 Glycosyltransferases (2.4)

Abstract

The present invention provides for a cell comprising a modified or altered enzymatic activity of a rice-diverged glycosyltransferase (GT).

Claims

1. A cell comprising a modified or altered enzymatic activity of a rice-diverged glycosyltransferase (GT).

2. The cell of claim 1 wherein the rice-diverged GT comprises an amino acid sequence that comprises an equal to or more than 80% sequence similarity with an amino acid sequence chosen from the group consisting of SEQ ID NOs:

1-269.

3. The cell of claim 2 wherein the rice-diverged GT comprises an amino acid sequence that comprises an equal to or more than 90% sequence similarity with an amino acid sequence chosen from the group consisting of SEQ ID NOs:

1-269.

4. The cell of claim 3 wherein the rice-diverged GT comprises an amino acid sequence that comprises an equal to or more than 95% sequence similarity with an amino acid sequence chosen from the group consisting of SEQ ID NOs:

1-269.

5. The cell of claim 4 wherein the rice-diverged GT comprises an amino acid sequence that comprises an equal to or more than 99% sequence similarity with an amino acid sequence chosen from the group consisting of SEQ ID NOs:

1-269.

6. The cell of claim 2 wherein the rice-diverged GT comprises an amino acid sequence that comprises an equal to or more than 80% sequence similarity with an amino acid sequence chosen from the group consisting of SEQ ID NOs:

1-30.

7. The cell of claim 6 wherein the rice-diverged GT comprises an amino acid sequence that comprises an equal to or more than 90% sequence similarity with an amino acid sequence chosen from the group consisting of SEQ ID NOs:

1-30.

8. The cell of claim 7 wherein the rice-diverged GT comprises an amino acid sequence that comprises an equal to or more than 95% sequence similarity with an amino acid sequence chosen from the group consisting of SEQ ID NOs:

1-30.

9. The cell of claim 8 wherein the rice-diverged GT comprises an amino acid sequence that comprises an equal to or more than 99% sequence similarity with an amino acid sequence chosen from the group consisting of SEQ ID NOs:

1-30.

10. The cell of claim 1 wherein the rice-diverged GT is highly expressed in the vegetative above ground plant tissue.

11. The cell of claim 1 wherein the expression of the rice-diverge GT is increased or reduced as compared to a wild-type cell.

12. The cell of claim 11 wherein the expression of the rice-diverge GT is reduced and the cell is knocked-out for the gene encoding the rice-diverge GT.

13. The cell of claim 1 wherein the cell is a plant cell.

14. The cell of claim 13 wherein the plant cell is a monocot plant cell.

15. The cell of claim 1, wherein the rice-diverged GT is involved in synthesis of cellulose or cell wall synthesis.

16. The cell of claim 15, wherein the rice-diverged GT is involved in synthesis of a type I wall-specific or enriched component.

17. The cell of claim 15, wherein the rice-diverged GT is involved in synthesis of a type II wall-specific or enriched component.

18. The cell of claim 15, wherein the rice-diverged GT is involved in synthesis of glucuronoarabinoxylan.

19. A method of identifying or determining a rice-diverged GT of a plant or cell, comprising:

(a) providing the amino acid sequence of a monocot gene not known to be rice-diverged GT, and (b) identifying the gene as lacking a dicot ortholog.

20. The method of claim 19 further comprising:

determining the expression level of the gene in vegetative above ground plant tissue.

21. The method of claim 20 further comprising:

constructing a plant cell that is reduced or increased in the expression of the gene.