Bio-driver apparatus for detecting a nucleic acid
First Claim
1. A bio-driver apparatus for detecting a nucleic acid comprising:
- a nucleic acid hybridization assay device comprising;
a cleavable signal element comprising;
a capture probe of a single strand having a complementary sequence to a target nucleic acid; and
a restriction probe of a single strand not being complementary to the target nucleic acid, wherein one end of the restriction probe is ligated to the capture probe; and
a solid support substrate attached to the other end of the restriction probe; and
a driver-body which contains a loader, a detector and a motor, wherein the solid support substrate is to be mounted and loaded onto the loader, the detector detects whether the cleavable signal elements are cleaved or not, and the motor rotates or moves the solid support substrate.
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Abstract
A cleavable signal element applicable to quantitative and qualitative assay devices, using a cleavable technique specifically responsive to a complementary double strand or single strand of nucleic acids, and a nucleic acid hybridization assay method and device using the cleavable signal element are provided. Using the cleavable technique responsive to the complementary double strand or single strand of nucleic acids, detection sensitivity to a target nucleic acid can be increased, and diagnosis and detection reliability can be improved twice through in-situ determinations. Through simultaneous single nucleotide polymorphism (SNP) detection and expression profile determination, more accurate diagnosis for many diseases can be achieved. The assay device can be easily modified to be suitable for detection with general laser-based detection systems such as CD-ROM readers. Information read from the assay device is digitized as software and transmitted to and received by doctors and patients through a computer network or wirelessly, which enables construction of remote diagnosis systems.
9 Citations
30 Claims
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1. A bio-driver apparatus for detecting a nucleic acid comprising:
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a nucleic acid hybridization assay device comprising; a cleavable signal element comprising;
a capture probe of a single strand having a complementary sequence to a target nucleic acid; and
a restriction probe of a single strand not being complementary to the target nucleic acid, wherein one end of the restriction probe is ligated to the capture probe; anda solid support substrate attached to the other end of the restriction probe; and a driver-body which contains a loader, a detector and a motor, wherein the solid support substrate is to be mounted and loaded onto the loader, the detector detects whether the cleavable signal elements are cleaved or not, and the motor rotates or moves the solid support substrate. - View Dependent Claims (2, 3, 4, 5, 6, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification