DNA-ARRAY-EQUIPPED CARTRIDGE, ANALYZER, AND METHOD FOR USING THE DNA-ARRAY-EQUIPPED CARTRIDGE

• US 20100144541A1
• Filed: 11/19/2009
• Published: 06/10/2010
• Est. Priority Date: 12/09/2008
• Status: Abandoned Application

• Current Assignee: NGK Insulators Limited
• Original Assignee: NGK Insulators Limited
• Inventors: Yamada, Kazunari, Murasato, Masahiro, Oribe, Akinobu
• Application Number:
  US12/621,771
• Publication Number:
  US 20100144541A1
• Time in Patent Office: Days
• US Class Current: 506/7
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/1805 Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0622 distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
• CPC Class Codes: B01L 2400/0644 rotary valves
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001

Abstract

Rotating a cartridge body 54 allows distribution ports and a combined distribution port provided in the cartridge body 54 and a channel inlet 53c provided in an upper surface of a ring array 53 to sequentially face a fluid port 30a of a reaction tank 30 independent of the cartridge body 54. Additionally, rotating the cartridge body 54 allows a plurality of DNA probes 53a to sequentially face a collimating lens 62a serving as a light detector independent of the cartridge body 54.

Claims

1. A DNA-array-equipped cartridge comprising:

a housing rotatable about a center axis;

a plurality of fluid containing spaces formed inside the housing and including a plurality of reagent containing spaces and a DNA array space, the reagent containing spaces holding fluids for preparation of target DNA, the DNA array space formed in a circumferential shape coaxial with the center axis and having a plurality of DNA probes spotted along the circumferential shape; and

a plurality of openings communicating with the corresponding fluid containing spaces, formed on an upper side of the housing, and arranged side-by-side along a circumference coaxial with the center axis,

wherein rotating the housing allows the plurality of openings to sequentially face a position setting a fluid port of a reaction tank independent of the housing, and allows the plurality of DNA probes to sequentially face a position setting a light detector independent of the housing.

2. The DNA-array-equipped cartridge according to claim 1, wherein the housing is formed in a substantially disk-like shape.

3. The DNA-array-equipped cartridge according to claim 1, wherein the plurality of DNA probes are spotted along a plurality of circumferential shapes coaxial with the center axis and having different diameters.

4. The DNA-array-equipped cartridge according to claim 1, further comprising a circular valve coaxial with the center axis of the housing, unrotatably secured, capable of supporting the reaction tank on an upper side of the circular valve, and having a through hole extending vertically therethrough from the fluid port of the reaction tank,

wherein rotating the housing allows the plurality of openings to sequentially face the through hole of the circular valve.

5. The DNA-array-equipped cartridge according to claim 1, further comprising a light guide configured to the position setting guide light to the light detector, the light being incident from the DNA probe facing the position setting the light detector.

6. The DNA-array-equipped cartridge according to claim 4, wherein the circular valve includes a light guide configured to guide light to the position setting the light detector, the light being incident from the DNA probe facing the position setting the light detector.

7. The DNA-array-equipped cartridge according to claim 5, wherein the light guide is a lens configured to collimate and guide light to a position setting the light detector, the light being incident from the DNA probe facing the light detector.

8. The DNA-array-equipped cartridge according to claim 6, wherein the light guide is a lens configured to collimate and guide light to a position setting the light detector, the light being incident from the DNA probe facing the light detector.

9. The DNA-array-equipped cartridge according to claim 1, further comprising a highly thermal-conductive member disposed opposite a position setting the light detector with respect to the DNA array space and made of carbon-containing resin or metal.

10. The DNA-array-equipped cartridge according to claim 8, further comprising a low-reflection ring disposed on the same side as a position setting the light detector with respect to the DNA array space, the low-reflection ring having a through portion communicating with the position setting the light detector and made of carbon-containing resin or metal.

11. The DNA-array-equipped cartridge according to claim 1,

wherein the plurality of fluid containing spaces include a column containing space and a waste liquid tank, the column containing space containing a column for purification of the target DNA, the waste liquid tank communicating with an upper part of the column containing space, and

wherein the plurality of openings include first and second openings communicating with the column containing space, the first opening communicating with a lower part of the column, the second opening communicating with an upper part of the column.

12. The DNA-array-equipped cartridge according to claim 1, wherein labeled markers are spotted at at least two predetermined positions in the DNA array space.

13. An analyzer comprising:

a holder for holding the DNA-array-equipped cartridge according to claim 1;

a rotator for rotating, about the center axis, the housing of the DNA-array-equipped cartridge held by the holder;

the reaction tank;

the light detector; and

a liquid transporter for transporting, through the corresponding openings, fluid held in the fluid containing spaces to the reaction tank, and fluid held in the reaction tank to the fluid containing spaces,

wherein when the housing of the DNA-array-equipped cartridge held by the holder is rotated by the rotator, the plurality of openings of the DNA-array-equipped cartridge sequentially face the fluid port of the reaction tank, and the plurality of DNA probes sequentially face the light detector.

14. A method for using the DNA-array-equipped cartridge according to claim 1, the method comprising the steps of:

(a) preparing the DNA-array-equipped cartridge in which fluids for preparation of the target DNA are held in the reagent containing spaces;

(b) preparing the reaction tank independent of the housing of the DNA-array-equipped cartridge and holding a sample from which the target DNA is prepared;

(c) rotating the housing to allow the openings of the reagent spaces to sequentially face the fluid port of the reaction tank, temporarily stopping the rotation of the housing in a state where the opening of each of the reagent spaces faces the reaction tank, transporting fluid between the reaction tank and the reagent space to prepare the target DNA, and eventually storing the target DNA in the reaction tank;

(d) rotating the housing to allow the opening of the DNA array space to face the fluid port of the reaction tank, causing the target DNA in the reaction tank to flow into the DNA array space, and causing the target DNA to react with each of the DNA probes; and

(e) rotating the housing and detecting light incident from each of the DNA probes subjected to the reaction by means of the light detector independent of the housing.