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Qualitative Differential Screening

  • US 20100144550A1
  • Filed: 11/19/2009
  • Published: 06/10/2010
  • Est. Priority Date: 03/11/1998
  • Status: Active Grant
First Claim
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1. A device for identifying at least one differentially spliced gene product, wherein said device comprisesa solid support material and single-stranded oligonucleotides of between 5 and 100 nucleotides in length attached to said support material,wherein said oligonucleotides comprise at least a first and a second oligonucleotide molecule arranged serially on the support material,wherein said first oligonucleotide molecule comprises a first sequence that is complementary to and specific for a first exon or exon-exon or exon-intron junction region of a first gene or RNA, and wherein said first sequence corresponds to a region of variability in at least one product of said first gene due to differential splicing, andwherein said second oligonucleotide molecule comprises a second sequence that is complementary to and specific for a second exon or exon-exon or exon-intron junction region of said first gene or RNA, and wherein said second sequence corresponds to a region of variability in at least one product of said first gene due to differential splicing,said device allowing, when contacted with a diverse population of nucleic acid molecules prepared from a biological sample under conditions allowing hybridisation to occur, the determination of the presence or absence of said differentially spliced gene product.

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