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GENOMIC SEQUENCING USING MODIFIED PROTEIN PORES AND IONIC LIQUIDS

  • US 20100148126A1
  • Filed: 11/24/2009
  • Published: 06/17/2010
  • Est. Priority Date: 11/26/2008
  • Status: Abandoned Application
First Claim
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1. A method of detecting the presence of one or more analytes in a sample, comprising the steps of:

  • dissolving the one or more analytes in the sample in water or a buffer solution comprising an ionic salt to form a solution;

    placing the solution in a cis compartment of a single-channel sensor;

    contacting the solution with a pore assembly comprising a genetically modified bacterial transmembrane protein toxin;

    applying an electrical potential to the single-channel sensor;

    determining an ionic current across the electrical potential;

    measuring one or more transient blockades in the ionic current; and

    comparing the transient blockades in the ionic current to one or more known transient current blockades to determine the identity of the one or more analytes.

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