Polypeptides of Strain Bacillus SP. P203

• US 20100151111A1
• Filed: 08/15/2006
• Published: 06/17/2010
• Est. Priority Date: 08/16/2005
• Status: Active Grant

• Current Assignee: Novozymes A/S (Novozymes)
• Original Assignee: Novozymes A/S (Novozymes)
• Inventors: Nielsen, Preben, Borchert, Martin, Wilting, Reinhard
• Granted Patent: US 8,119,387 B2
• Time in Patent Office: Days
• US Class Current: 426/656
• CPC Class Codes: C12N 9/2468 acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
• CPC Class Codes: C12N 9/248 Xylanases
• CPC Class Codes: C12N 9/54 bacteria being Bacillus
• CPC Class Codes: C12N 9/88 Lyases (4.)
• CPC Class Codes: C12Y 302/01008 Endo-1,4-beta-xylanase (3.2.1.8)
• CPC Class Codes: C12Y 305/02006 Beta-lactamase (3.5.2.6)
• CPC Class Codes: C12Y 402/01001 Carbonate dehydratase (4.2.1.1), i.e. carbonic anhydrase

Abstract

A new strain Bacillus sp. P203 (Bacillus plakortiensis) is disclosed. Isolated mature functional polypeptide which is obtainable from the bacterium strain Bacillus sp. P203 deposited under accession number DSM 17419 are disclosed.

Claims

1-26. -26. (canceled)

27. A functional polypeptide encoded by a polynucleotide comprised in the genome of a strain of Bacillus plankortiensis deposited under accession number DSM 17419.

28. An isolated mature functional polypeptide obtainable from the Bacillus sp. P203 strain deposited under accession number DSM 17419.

29. The polypeptide of claim 28 selected from the group consisting of:

(a) a polypeptide comprising an amino acid sequence which has at least 70% identity with a sequence selected from the group consisting of SEQ ID NO;

2, SEQ ID NO;

4, SEQ ID NO;

6, SEQ ID NO;

8, SEQ ID NO;

10 and SEQ ID NO;

12;

(b) a polypeptide which is encoded a nucleotide sequence which hybridizes under high stringency conditions with a polynucleotide probe selected from the group consisting of

(i) the complementary strand to a nucleotide sequence selected from the group consisting of SEQ ID NO;

1, SEQ ID NO;

3, SEQ ID NO;

5, SEQ ID NO;

7, SEQ ID NO;

9 and SEQ ID NO;

11 encoding a mature polypeptide; and

(ii) the complementary strand to the cDNA sequence contained in a nucleotide sequences selected from the group of SEQ ID NO;

1, SEQ ID NO;

3, SEQ ID NO;

5, SEQ ID NO;

7, SEQ ID NO;

9 and SEQ ID NO;

11 encoding a mature polypeptide; and

(c) a fragment of a mature polypeptide selected from the group consisting of SEQ ID NO;

2, SEQ ID NO;

4, SEQ ID NO;

6, SEQ ID NO;

8, SEQ ID NO;

10 and SEQ ID NO;

12;

wherein the polypeptide has a function of the corresponding mature polypeptides comprised in SEQ ID NO;

2, SEQ ID NO;

4, SEQ ID NO;

6, SEQ ID NO;

8, SEQ ID NO;

10 and SEQ ID NO;

12.

30. The polypeptide of claim 28, wherein the polypeptide is an enzyme having a function selected from the group consisting of xylanase, serine protease, carbonic anhydrase, rhamnogalacturonan lyase and galactanase.

31. The enzyme of claim 30 selected from the group consisting of:

(a) an enzyme having an amino acid sequence which has at least 70% identity with an amino acid sequence selected from a mature enzyme comprised in SEQ ID NO;

2, SEQ ID NO;

4, SEQ ID NO;

6, SEQ ID NO;

8, SEQ ID NO;

10 and SEQ ID NO;

12;

(b) an enzyme which is encoded by a nucleotide sequence which hybridize under high stringency conditions with a polynucleotide probe selected from the group consisting of

(i) the complementary strand to a nucleotide sequence selected from the group of regions of SEQ ID NO;

1, SEQ ID NO;

3, SEQ ID NO;

5, SEQ ID NO;

SEQ NO;

9 and SEQ ID NO;

11 encoding the mature enzyme,

(ii) the complementary strand to the cDNA sequence contained in a nucleotide sequences selected from regions of SEQ ID NO;

1, SEQ ID NO;

3, SEQ ID NO;

5, SEQ ID NO;

7, SEQ ID NO;

9 and SEQ ID NO;

11 encoding the mature polypeptide;

(c) a fragment of the mature enzyme comprised in SEQ ID NO;

2, SEQ ID NO;

4, SEQ ID NO;

6, SEQ ID NO;

8, SEQ ID NO;

10 and SEQ ID NO;

12;

wherein the enzyme has a function of the corresponding mature polypeptides comprised in SEQ ID NO;

2, SEQ ID NO;

4, SEQ ID NO;

6, SEQ ID NO;

8, SEQ ID NO;

10 and SEQ ID NO;

12.

32. The polypeptide of claim 28, wherein the polynucleotide encoding the polypeptide consists of a nucleotide sequence selected from the group of regions of SEQ ID NO:

1, SEQ ID NO;

3, SEQ ID NO;

5, SEQ ID NO;

7, SEQ ID NO;

9 and SEQ ID NO;

11 encoding a mature polypeptide or a sequence differing there from by virtue of the degeneracy of the genetic code.

34. A composition comprising a polypeptide of claim 28.

35. The composition of claim 34, further comprising one or more additional enzymes.

36. The composition of claim 34, further comprising a cereal or grain product.

37. The composition of claim 34, further comprising a polysaccharide or a mixture of polysaccharides.

38. A detergent composition comprising a polypeptide of claim 28 and a surfactant.

39. A polynucleotide having a nucleotide sequence which encodes for a polypeptide of claim 28.

40. A nucleic acid construct comprising the nucleotide sequence defined in claim 39 operably linked to one or more control sequences that direct the production of the polypeptide in a host cell.

41. A recombinant expression vector comprising the nucleic acid construct of claim 40.

42. A recombinant host cell comprising the nucleic acid construct of claim 40.

43. A method for producing a polypeptide, comprising:

(a) cultivating a recombinant host cell of claim 42 under conditions conducive for production of the polypeptide; and

(b) recovering the polypeptide.

33. An isolated enzyme selected from the group consisting of:

(a) an enzyme comprising an amino acid sequence which has at least 70% identity with the amino acid sequence of a mature enzyme selected from the group consisting of xylanase, serine protease, carbonic anhydrase, rhamnogalacturonan lyase and galactanase secreted from the strain of strain Bacillus sp. P203 deposited under DSM 17419;

(b) a polypeptide which is encoded by a nucleotide sequence which hybridize under high stringency conditions with a polynucleotide probe selected from the group consisting of;

(i) the complementary strand to a nucleotide sequence comprised in the strain of strain Bacillus sp. P203 deposited under accession number DSM 17419 encoding a mature enzyme selected from the group consisting of xylanase, serine protease, carbonic anhydrase, rhamnogalacturonan lyase and galactanase secreted from that strain;

(ii) the complementary strand to the cDNA sequence contained in a nucleotide sequences comprised in the strain of strain Bacillus sp. P203 deposited under accession number DSM 17419 encoding a mature enzyme selected from the group consisting of xylanase, serine protease, carbonic anhydrase, rhamnogalacturonan lyase and galactanase secreted from that strain;

(c) a fragment of a mature enzyme selected from the group consisting of xylanase, serine protease, carbonic anhydrase, rhamnogalacturonan lyase and galactanase secreted from the Bacillus sp. P203 strain deposited under accession number DSM 17419;

wherein the enzyme have a function selected from xylanase, serine protease, carbonic anhydrase, rhamnogalacturonan lyase and galactanase.