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MICROFLUIDIC APPARATUS, SYSTEMS, AND METHODS FOR PERFORMING MOLECULAR REACTIONS

  • US 20100151454A1
  • Filed: 10/22/2008
  • Published: 06/17/2010
  • Est. Priority Date: 03/31/2004
  • Status: Abandoned Application
First Claim
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1. A method to detect a nucleotide, comprising:

  • a) restraining movement of a nucleic acid molecule attached to a single particle using a restriction barrier located within a first channel;

    b) contacting the nucleic acid molecule with an exonuclease to release the nucleotide, wherein the nucleotide is a terminal nucleotide; and

    c) identifying the released nucleotide by associating the released nucleotide with a surface enhanced Raman spectroscopy-active surface, irradiating the released nucleotide with a detection laser beam and measuring Raman emission from the irradiated nucleotide, thereby detecting the nucleotide.

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