COMPOSITIONS AND METHODS FOR PREPARING OLIGONUCLEOTIDE SOLUTIONS
First Claim
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1. A method of generating a pool of oligonucleotides comprising:
- (a) providing a substrate and at least first and second different oligonucleotides linked to said substrate through first and second cleavable linkers, respectively; and
(b) cleaving said first and second linkers, thereby releasing said first and second oligonucleotides from said substrate thereby generating a pool of oligonucleotides comprising said first and second oligonucleotides.
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Abstract
The present invention is directed to methods and compositions for generating a pool of oligonucleotides. The invention finds use in preparing a population or subpopulations of oligonucleotides in solution. The pool of oligonucleotides finds use in a variety of nucleic acid detection and/or amplification assays.
137 Citations
26 Claims
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1. A method of generating a pool of oligonucleotides comprising:
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(a) providing a substrate and at least first and second different oligonucleotides linked to said substrate through first and second cleavable linkers, respectively; and (b) cleaving said first and second linkers, thereby releasing said first and second oligonucleotides from said substrate thereby generating a pool of oligonucleotides comprising said first and second oligonucleotides. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for generating a pool of oligonucleotides, said method comprising:
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(a) providing an array comprising a substrate and a population of oligonucleotides, said population comprising at least first and second subpopulations comprising at least first and second different oligonucleotides, respectively, said first and second oligonucleotides being immobilized to first and second beads, respectively, through first and second cleavable linkers, respectively, said first and second beads being distributed on said substrate, and (b) cleaving said first and second linkers, thereby releasing said first and second subpopulations from said first and second beads, thereby generating a pool of oligonucleotides comprising said first and second oligonucleotides. - View Dependent Claims (12, 13, 14)
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15. A method for generating a pool of oligonucleotides, said method comprising:
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(a) providing an array comprising a substrate and a population of oligonucleotides, said population comprising at least first and second subpopulations comprising at least first and second different oligonucleotides of known sequence, said first and second oligonucleotides being immobilized directly to a chip through first and second cleavable linkers, respectively; and (b) cleaving said first and second linkers, thereby releasing said first and second subpopulations from said chip, thereby generating a pool of oligonucleotides comprising said first and second oligonucleotides. - View Dependent Claims (16)
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17. A composition comprising:
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(a) a substrate; and (b) at least first and second different oligonucleotides of known sequence linked to said substrate through first and second cleavable linkers, respectively. - View Dependent Claims (18, 19, 20, 21, 22, 23)
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24. A kit comprising:
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(a) a substrate; (b) at least first and second different oligonucleotides of known sequence linked to said substrate through first and second cleavable linkers, respectively; and (c) at least one linker cleaving agent. - View Dependent Claims (25, 26)
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Specification