Nucleic Acids Useful in the Manufacture of Oil
First Claim
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1. A recombinant nucleic acid molecule comprising a promoter that is upregulated at least 3-fold in a cell of the genus Prototheca when the extracellular environment changes from containing at least 5 mM nitrogen to containing no nitrogen.
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Abstract
Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.
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Citations
13 Claims
- 1. A recombinant nucleic acid molecule comprising a promoter that is upregulated at least 3-fold in a cell of the genus Prototheca when the extracellular environment changes from containing at least 5 mM nitrogen to containing no nitrogen.
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3. A nucleic acid vector comprising an expression cassette comprising (a) a promoter that is active in a cell of the genus Prototheca;
- and (b) a coding sequence in operable linkage with the promoter wherein the coding sequence contains the most or second most preferred codons of Table 1 for at least 20% of the codons of the coding sequence.
- View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11)
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12. (canceled)
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13. A recombinant nucleic acid encoding a protein with at least 90% amino acid identity to one or both of SEQ ID NOs:
- 136-137.
Specification