DEFINITIVE ENDODERM
First Claim
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1. An in vitro cell culture comprising human ectoderm cells and human definitive endoderm cells, wherein said definitive endoderm cells are multipotent cells that can differentiate into cells of the gut tube or organs derived therefrom, and wherein said ectoderm cells comprise less than 5% of the cells in said cell culture.
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Abstract
Disclosed herein are cell cultures comprising definitive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified definitive endoderm cells as well as methods for enriching, isolating and purifying definitive endoderm cells from other cell types.
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15 Claims
- 1. An in vitro cell culture comprising human ectoderm cells and human definitive endoderm cells, wherein said definitive endoderm cells are multipotent cells that can differentiate into cells of the gut tube or organs derived therefrom, and wherein said ectoderm cells comprise less than 5% of the cells in said cell culture.
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