Method of Assaying Antigen and Kit to be Used therein

• US 20100151587A1
• Filed: 11/10/2006
• Published: 06/17/2010
• Est. Priority Date: 11/18/2005
• Status: Active Grant

• Current Assignee: Nitto Boseki Company Limited
• Original Assignee: Nitto Boseki Company Limited
• Inventors: Katayama, Katsuhiro, Sato, Yoshiro, Kojima, Ryo
• Granted Patent: US 7,981,690 B2
• Time in Patent Office: Days
• US Class Current: 436/501
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: G01N 33/545 Synthetic resin

Abstract

By using an antibody which is covalently bonded to a water soluble polymer as an antibody to be used in a competitive immunoagglutination assay in a homogeneous system, a protein antigen at a high concentration can be accurately assayed in an undiluted system without resorting to dilution.

Claims

1. A method of assaying an antigen by a competitive immunoagglutination assay in a homogeneous system comprising the steps of:

mixing a sample containing an antigen to be assayed, an antibody against the antigen, and fine particles carrying the same antigen as the antigen to be assayed or an analogue of the antigen, which analogue may undergo an antigen-antibody reaction with the antibody;

competing an antigen-antibody reaction of the antibody between the antigen in the sample and the same antigen as the antigen to be assayed or the analogue of the antigen, both of which are carried on fine particles; and

quantitatively determining the antigen to be assayed based on the degree of agglutination as a result of the antigen-antibody reaction of the antibody with the same antigen as the antigen to be assayed or the analogue of the antigen, both of which are carried on fine particles,

characterized in that the antibody is covalently bonded to a water soluble polymer.

2. The method of assay according to claim 1, wherein the water soluble polymer is polyethylene glycol, polypropylene glycol homopolymer, or polyvinyl alcohol.

3. The method of assay according to claim 1, wherein the water soluble polymer is polyethylene glycol.

4. The method of assay according to claim 3, wherein the antigen to be assayed is a protein present in a biological sample at a high concentration.

5. The method of assay according to claim 4, wherein the antigen to be assayed is an albumin.

6. The method of assay according to claim 5, wherein the assay is conducted without diluting a sample containing an antigen to be assayed.

8. The kit according to claim 6, wherein the water soluble polymer is polyethylene glycol, polypropylene glycol homopolymer, or polyvinyl alcohol.

10. The method of assay according to claim 1, wherein the antigen to be assayed is a protein present in a biological sample at a high concentration.

11. The method of assay according to claim 10, wherein the antigen to be assayed is an albumin.

12. The method of assay according to claim 11, wherein the assay is conducted without diluting a sample containing an antigen to be assayed.

13. The method of assay according to claim 2, wherein the antigen to be assayed is a protein present in a biological sample at a high concentration.

14. The method of assay according to claim 13, wherein the antigen to be assayed is an albumin.

15. The method of assay according to claim 14, wherein the assay is conducted without diluting a sample containing an antigen to be assayed.

16. The method of assay according to claim 1, wherein the assay is conducted without diluting a sample containing an antigen to be assayed.

17. The method of assay according to claim 2, wherein the assay is conducted without diluting a sample containing an antigen to be assayed.

18. The method of assay according to claim 3, wherein the assay is conducted without diluting a sample containing an antigen to be assayed.

19. The method of assay according to claim 10, wherein the assay is conducted without diluting a sample containing an antigen to be assayed.

20. The method of assay according to claim 13, wherein the assay is conducted without diluting a sample containing an antigen to be assayed.

7. A kit for assaying an antigen by a competitive immunoagglutination assay in a homogeneous system, including an antibody against an antigen to be assayed, which antibody may be covalently bonded to a water soluble polymer, and fine particles carrying the same antigen as the antigen to be assayed or an analogue of the antigen, which analogue may undergo an antigen-antibody reaction with the antibody.

9. The kit according to claim 7, wherein the water soluble polymer is polyethylene glycol.