SUB-MICROMETER FLUIDIC CHANNEL FOR MEASURING PHOTON EMITTING ENTITIES
First Claim
1. A device comprising:
- a channel having a narrow detection portion sized to promote single molecule flow through the channel;
a light source to focus light substantially uniformly across a width of the detection portion;
means for moving photon emitting entities through the detection portion; and
a detector for detecting photon emissions from the photon emitting entities.
2 Assignments
0 Petitions
Accused Products
Abstract
A nanofluidic channel fabricated in fused silica with an approximately 500 nm square cross section was used to isolate, detect and identify individual quantum dot conjugates. The channel enables the rapid detection of every fluorescent entity in solution. A laser of selected wavelength was used to excite multiple species of quantum dots and organic molecules, and the emission spectra were resolved without significant signal rejection. Quantum dots were then conjugated with organic molecules and detected to demonstrate efficient multicolor detection. PCH was used to analyze coincident detection and to characterize the degree of binding. The use of a small fluidic channel to detect quantum dots as fluorescent labels was shown to be an efficient technique for multiplexed single molecule studies. Detection of single molecule binding events has a variety of applications including high throughput immunoassays.
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Citations
38 Claims
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1. A device comprising:
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a channel having a narrow detection portion sized to promote single molecule flow through the channel; a light source to focus light substantially uniformly across a width of the detection portion; means for moving photon emitting entities through the detection portion; and a detector for detecting photon emissions from the photon emitting entities. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A device comprising:
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a channel having a nano-channel detection portion for single molecule flow; a light source that focuses light substantially uniformly across a width of the detection portion; a driver that moves photon emitting entities through the detection portion; and a detector that detects emissions from the photon emitting entities. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A device comprising:
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an array of channels, each having a nano-channel detection portion for single molecule flow, wherein the channels are formed on a substrate and covered with a wafer; a light source that focuses light substantially uniformly across a width of the detection portion of the channels; a driver that moves photon emitting entities through the detection portions; and a detector that detects emissions from the photon emitting entities. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
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37. A device comprising:
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a channel having a nano-channel detection portion; a light source that focuses light substantially uniformly across a width of the detection portion; a driver that moves photon emitting entities through the detection portion; and a detector that detects emissions from the photon emitting entities. - View Dependent Claims (38)
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Specification