METHOD FOR EXTRACTING AND SELECTING CELLS
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Abstract
Method for selecting cells, comprising at least one at least partially combined step of enzymatic digestion and of selection in culture.
10 Citations
115 Claims
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1-44. -44. (canceled)
- 45. A method of cell selection in a culture of cells, the method comprising at least one at least partially combined step of enzymatic digestion and selection in culture.
- 70. A method for selecting muscle cells by a freezing step in the presence of a compound chosen from a group comprising sugars, trehalose, glycine, HES (hydroxyethyl starch), glycerol and arbutin.
- 95. A culture medium that is substantially free of insulin, comprising at least dexamethasone, selenium, and one or more compounds chosen from a group comprising ascorbic acid 2-phosphate, ascorbic acid and mixtures thereof.
- 102. A culture medium comprising at least dexamethasone, selenium, ascorbic acid 2-phosphate and ascorbic acid.
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108. A culture medium comprising at least dexamethasone at a concentration of approximately 5×
- 10−
9 M, ascorbic acid 2-phosphate at a concentration of approximately 1 mM, ascorbic acid at a concentration of 0.252 mM and sodium selenite at a concentration of 250 nM. - View Dependent Claims (109, 110)
- 10−
- 111. A cell preservation medium comprising albumin, trehalose and DMSO.
Specification