METHODS AND SYSTEMS FOR GENETIC ANALYSIS OF FETAL NUCLEATED RED BLOOD CELLS
First Claim
1. A method for determining genetic status of a fetus, said method comprising;
- providing a sample of maternal blood;
enriching nucleated red blood cells including both fetal and maternal nucleated red blood cells in the maternal blood sample;
differentiating the nucleated red blood cells from all other blood cells in the enriched sample; and
screening at least a portion of the differentiated nucleated red blood cells for a genetic status which may be possessed by the fetus but not by the mother.
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Abstract
Methods for determining genetic status of a fetus from a sample of maternal blood comprise enriching nucleated red blood cells in the sample, including both fetal and maternal nucleated red blood cells. The nucleated red blood cells are then differentiated from all other blood cells in the enriched sample, and the nucleated red blood cells genetically screened to determine the genetic status. The nucleated red blood cells may be differentiated by immobilizing all enriched blood cells on a solid phase and locating the nucleated red blood cells for interrogation. Optionally, the nucleated red blood cells may be sorted and separated from other enriched blood cells in a liquid phase.
120 Citations
47 Claims
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1. A method for determining genetic status of a fetus, said method comprising;
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providing a sample of maternal blood; enriching nucleated red blood cells including both fetal and maternal nucleated red blood cells in the maternal blood sample; differentiating the nucleated red blood cells from all other blood cells in the enriched sample; and screening at least a portion of the differentiated nucleated red blood cells for a genetic status which may be possessed by the fetus but not by the mother. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
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44. A method for identifying an increased risk of abnormal pregnancy in a pregnant female, said method comprising;
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providing a sample of maternal blood; enriching nucleated red blood cells including both fetal and maternal nucleated red blood cells in the maternal blood sample; differentiating the nucleated red blood cells from all other blood cells in the enriched sample; and determining the number of nucleated red blood cells in the sample, wherein an elevated number of nucleated red blood cells in comparison to a threshold number indicates an increased risk of abnormal pregnancy in the pregnant female. - View Dependent Claims (45, 46, 47)
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Specification