Colony-forming unit cell of human chorion and method to obtain and use thereof

• US 20100166716A1
• Filed: 12/03/2009
• Published: 07/01/2010
• Est. Priority Date: 12/29/2008
• Status: Active Grant

• Current Assignee: Frans A. Kuypers, Vladimir B. Serikov
• Original Assignee: Frans A. Kuypers, Vladimir B. Serikov
• Inventors: Serikov, Vladimir B., Kuypers, Frans A.
• Granted Patent: US 8,771,677 B2
• Time in Patent Office: Days
• US Class Current: 424/93.700
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 35/50 Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C12N 5/0605 Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
• CPC Class Codes: C12N 5/0607 Non-embryonic pluripotent stem cells, e.g. MASC induced pluripotent stem cells C12N5/0696

Abstract

The present invention features colony-forming unit cells derived from the chorion of human placenta and describes compositions and methods for the uses of chorionic cells and their products for therapeutic purposes based upon production and release of multiple growth factors and cytokines by these cells stimulating tissue regeneration independent of engraftment, as well as differentiation into a specific cell type.

Claims

1. Colony-forming unit cells derived from chorion of human term placentas which are characterized by:

I) expression of protein ACBD6,

II) low telomerase activity and ability to be propagated over at least 50 doublings in vitro culture without substantial alteration of karyotype,

III) expression of a combination of at least one of the markers from each of;

iv) CD-90, SSEA-3, TRA-1-60;

v) one or more cytosolic polypeptides selected from Nanog, neurofilament family, Nestin, or one or more secreted polypeptides selected from family of fibroblast growth factors, hepatocyte growth factors, keratinocyte growth factors, angiopoietins;

vi) nuclear peptide Oct-4.

IV) lack the ability of spontaneous differentiation into lineages of 3 germ layers under standard culture conditions, unless specifically stimulated, or lack of ability for formation of teratomas in humans or immuno-compromised animals,

V) ability to differentiate under appropriate conditions into lineages of all 3 germ layers.

2. Colony-forming unit cells of claim 1, capable of forming spherical three-dimensional structures in culture but not embryobodies.

3. Colony-forming unit cells of claim 1 wherein cells are multipotent or pluripotent stem cells.

4. Colony-forming unit cells of claim 1 wherein cells are capable of repopulating organs of humans or immunodeficient animals without formation of teratomas.

5. Colony-forming unit cells of claim 1 and a pharmaceutically acceptable carrier.

6. Colony-forming unit cells of claim 1 and an effective amount of any other cell population and a pharmaceutically acceptable carrier.

7. Colony-forming unit cells of claim 1 and a tissue-engineered construct comprising a biocompatible polymer scaffold.

10. A method of enhancing regeneration of diseased tissue of organs, by local or systemic delivery of cells of claim 1.

11. A method of claim 10 where the diseased organ is heart.

12. A Method of claim 10 where the diseased organ in spinal cord.

13. A method of claim 10 where the diseased organ is striated muscle.

14. A method of claim 10 where the diseased organ is peripheral nerve.

8. A method to obtain cells colony-forming unit cells of placental chorion based upon selection of non-adherent colony-forming cells from culture of cell populations.

9. A method of claim 8 of based upon selection of cells expressing surface marker ACBD6.