REDUCTION OF CARBON DIOXIDE EMISSION DURING ISOPRENE PRODUCTION BY FERMENTATION
First Claim
1. Cells comprising a heterologous nucleic acid that encodes an isoprene synthase polypeptide in operable combination with a promoter wherein the cells are in culture medium comprising one or more uncommon carbon sources and wherein the ratio of carbon dioxide emitted per isoprene produced in off gas from the cultured cells is less than 500.
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Abstract
The present invention provides methods for increasing the amount of isoprene produced by cultured cells with only a minimal increase in carbon dioxide emitted, thereby resulting in process having a greater yield of isoprene relative to carbon dioxide. In addition, the present invention provides compositions that include the cultured cells or isoprene produced there from.
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17 Claims
- 1. Cells comprising a heterologous nucleic acid that encodes an isoprene synthase polypeptide in operable combination with a promoter wherein the cells are in culture medium comprising one or more uncommon carbon sources and wherein the ratio of carbon dioxide emitted per isoprene produced in off gas from the cultured cells is less than 500.
Specification