Novel Boronate Complex and Its Use in a Glucose Sensor

• US 20100167416A1
• Filed: 11/08/2006
• Published: 07/01/2010
• Est. Priority Date: 11/08/2005
• Status: Active Grant

• Current Assignee: Smart Holograms Ltd., Cambridge Enterprise Limited (University of Cambridge)
• Original Assignee: Cambridge Enterprise Limited (University of Cambridge)
• Inventors: Horgan, Adrian Martin, Lee, Mei-Ching, Medlock, Kathryn Elizabeth Sorrell, Lowe, Christopher Robin, Blyth, Jeffrey, Kabilan, Satyamoorthy
• Granted Patent: US 8,334,140 B2
• Time in Patent Office: Days
• US Class Current: 436/501
• CPC Class Codes: G01N 21/453 Holographic interferometry for dimensional measurements G01B9/021 - G01B9/029
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: G02B 5/32 Holograms used as optical elements
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

A sensor comprises respective acceptor and donor compounds immobilised in or on a matrix including a glucose-binding boronate and a cationic species, whereby the spacing between the acceptor and donor compounds is reduced in the presence of glucose. For example a holographic sensor comprises a glucose-binding boronate and a cationic species held within the sensor.

Claims

1-31. -31. (canceled)

32. A sensor comprising respective acceptor and donor compounds immobilised in or on a matrix including a glucose-binding boronate and a quaternary ammonium compound, whereby the spacing between the acceptor and donor compounds is reduced in the presence of glucose.

33. The sensor according to claim 32, wherein the acceptor and donor compounds are a FRET pair.

34. The sensor according to claim 32, which comprises a fluor and a quencher pair.

35. The sensor according to claim 32, which is a holographic sensor.

43. An article comprising a sensor according to claim 32, which is a transaction card, banknote, passport, identification card, smart card, driving license, share certificate, bond, cheque, cheque card, tax banderole, gift voucher, postage stamp, rail or air ticket, telephone card, lottery card, event ticket, credit or debit card, business card, or an item used in consumer, brand or product protection for the purpose of distinguishing genuine products from counterfeit products or identifying stolen products.

44. An article comprising a sensor according to claim 32, which is a test strip, chip, cartridge, swab, tube, pipette, contact lens, sub-conjuctival implant, sub-dermal implant, breathalyser, catheter or a fluid sampling or analysis device.

45. A transferable holographic film comprising a sensor according to claim 32.

47. The sensor, according to claim 32, which has an optical filter thereon.

36. A holographic sensor comprising a glucose-binding boronate and a quaternary ammonium compound held within the sensor.

37. The sensor according to claim 36, further comprising means for producing an interference effect when illuminated with laser light.

38. The sensor according to claim 37, wherein the means comprises a depolarising layer.

39. The sensor according to claim 36, which has an optical filter thereon.

40. The sensor according to claim 39, wherein the optical filter is a bandpass filter.

49. An article comprising a sensor according to claim 36, which is a transaction card, banknote, passport, identification card, smart card, driving license, share certificate, bond, cheque, cheque card, tax banderole, gift voucher, postage stamp, rail or air ticket, telephone card, lottery card, event ticket, credit or debit card, business card, or an item used in consumer, brand or product protection for the purpose of distinguishing genuine products from counterfeit products or identifying stolen products.

41. A method of detection of an analyte in a sample, which comprises contacting the sample with the medium of a sensor and detecting any change of the optical characteristic wherein said sensor comprises respective acceptor and donor compounds immobilised in or on a matrix including a glucose-binding boronate and a quaternary ammonium compound, whereby the spacing between the acceptor and donor compounds is reduced in the presence of glucose.

42. The method according to claim 41, wherein the analyte is a chemical, biochemical or biological species.

46. A method of enhancing the security of an article, which comprises transferring onto the article a transferable holographic film comprising a sensor comprising respective acceptor and donor compounds immobilised in or on a matrix including a glucose-binding boronate and a quaternary ammonium compound, whereby the spacing between the acceptor and donor compounds is reduced in the presence of glucose.

48. A method of detection of an analyte in a sample, which comprises contacting the sample with the medium of a sensor, and detecting any change of the optical characteristic wherein the sensor comprises respective acceptor and donor compounds immobilised in or on a matrix including a glucose-binding boronate and a quaternary ammonium compound, whereby the spacing between the acceptor and donor compounds is reduced in the presence of glucose.