MICROARRAY CHIP AND METHOD OF FABRICATING FOR THE SAME

• US 20100167950A1
• Filed: 12/30/2009
• Published: 07/01/2010
• Est. Priority Date: 12/31/2008
• Status: Abandoned Application

• Current Assignee: National Health Research Institutes
• Original Assignee: National Health Research Institutes
• Inventors: Chen, Po-Cheng, Juang, Jyh-Lyh, Huang, Yi-You
• Application Number:
  US12/649,993
• Publication Number:
  US 20100167950A1
• Time in Patent Office: Days
• US Class Current: 506/9
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00509 Microcolumns
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00743 Cells
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 33/5008 for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics

Abstract

The present invention provides a microarray chip for use in the analysis of various sample types. The microarray chips disclosed herein generally comprise a substrate covered with a coating material comprising a photoresist material, wherein the coating material is patterned to comprise a plurality of microstructures such as microwells and/or microcolumns. Methods for preparing and utilizing the microarray chips of the invention are further provided. The microarray chips of the instant invention find particular use in high-throughput assays.

Claims

1. A microarray chip comprising:

(a) a substrate; and

(b) a coating material covering the substrate, wherein the coating material comprises at least one commercially available photoresist material and is patterned to comprise a plurality of microstructures on the substrate.

2. The microarray chip of claim 1, wherein the substrate is quartz.

3. The microarray chip of claim 1, wherein the substrate is glass.

4. The microarray chip of claim 1, wherein the at least one commercially available photoresist material comprises NANO™

SU-8 2-15, NANO™

SU-8 50-100, NANO™

SU-8 2000 series photoresist, NANO™

SU-8 3000 series photoresist and KMPR®

1000 series photoresist films.

5. The microarray chip of claim 1, wherein the microstructures are microwells.

6. The microarray chip of claim 1, wherein the microstructures are microcolumns.

7. The microarray chip of claim 6, wherein the microcolumns further comprises micromachined microcolumns.

8. The microarray chip of claim 6, wherein the microcolumns further comprises microelectromechanical systems (MEMS) fabricated microcolumns.

9. The microarray chip of claim 1, wherein the microstructures include a combination of microwells on one surface of the substrate and microcolumns on another surface of the substrate.

10. A platform for analyzing binding of a probe to a sample comprising:

(a) an array chip comprising at least one substrate and a coating material covering the substrate, wherein the coating material comprises at least one commercially available photoresist material and is patterned to comprise a plurality of microwells;

(b) a means for applying the probe to the sample in the microwells; and

(c) a means for detecting any binding of the probe and the sample and any phenotypic change resulting from binding of the probe and the sample.

11. The platform of claim 10, wherein the at least one commercially available photoresist material comprises NANO™

SU-8 2-15, NANO™

SU-8 50-100, NANO™

SU-8 2000 series photoresist, NANO™

SU-8 3000 series photoresist and KMPR®

1000 series photoresist films.

12. The platform of claim 10, wherein the substrate is quartz.

13. The platform of claim 10, wherein the substrate is glass.

14. The platform of claim 10, wherein the means for applying the probe comprises a plurality of microcolumns.

15. A drug screening method comprising:

(a) providing a microarray chip, wherein the microarray chip comprises a substrate and a coating material covering the substrate, wherein the coating material comprises at least one commercially available photoresist material and is patterned to comprise a plurality of microwells;

(b) culturing a target cell in the microwells;

(c) dispensing a candidate drug into the microwells; and

(d) detecting any binding of the candidate drug to the target cell and any phenotypic change resulting from binding of the candidate drug and the target cells.

16. The method of claim 15, wherein the at least one commercially available photoresist material comprises NANO™

SU-8 2-15, NANO™

SU-8 50-100, NANO™

SU-8 2000 series photoresist, NANO™

SU-8 3000 series photoresist and KMPR®

1000 series photoresist films.

17. The method of claim 15, wherein the candidate drug is dispensed from a plurality of microcolumns on the substrate.

18. A method for analyzing binding of a probe to a sample comprising:

(a) providing a microarray chip, wherein the microarray chip comprises a substrate and a coating material covering the substrate, wherein the coating material comprises at least one commercially available photoresist material and is patterned to comprise a plurality of microwells;

(b) applying the probe to the sample in the microwells; and

(c) detecting any binding of the probe to the sample and any phenotypic change resulting from binding of the probe to the sample.

19. The method of claim 18, wherein the at least one commercially available photoresist material comprises NANO™

SU-8 2-15, NANO™

SU-8 50-100, NANO™

SU-8 2000 series photoresist, NANO™

SU-8 3000 series photoresist and KMPR®

1000 series photoresist films.

20. The method of claim 18, wherein the probe is applied from a plurality of microcolumns on one surface of the substrate.