Novel Branchiostoma Derived Fluorescent Proteins
First Claim
1. An isolated Branchiostoma fluorescent protein, characterized by having a cellular fluorescence that is at least about five times greater than the cellular fluorescence of a GFP protein that fluoresces at normal conditions in cells, and having a chromophore region comprising amino acid sequence GYG.
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Accused Products
Abstract
The present invention provides compositions, combinations, methods, sequences and kits for use of novel fluorescent proteins derived from the genus Branchiostoma. Specifically, polynucleotide and polypeptide sequences encoding fluorescent proteins isolated from Branchiostoma floridae, including harmonized sequences, which permit enhanced expression of the encoded polypeptides in mammalian cells in vivo are provided.
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Citations
29 Claims
- 1. An isolated Branchiostoma fluorescent protein, characterized by having a cellular fluorescence that is at least about five times greater than the cellular fluorescence of a GFP protein that fluoresces at normal conditions in cells, and having a chromophore region comprising amino acid sequence GYG.
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4. An isolated Branchiostoma fluorescent protein, which has a polypeptide sequence that is selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
29, SEQ ID NO;
30, SEQ ID NO;
31, SEQ ID NO;
38, and proteins having a polypeptide sequence that is at least 70% identity to the listed sequences. - View Dependent Claims (5)
- 1, SEQ ID NO;
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13. (canceled)
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14. (canceled)
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15. (canceled)
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16. (canceled)
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17. (canceled)
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18. (canceled)
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19. (canceled)
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20. (canceled)
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21. An isolated fluorescent polypeptide, the amino acid sequence of which comprises a sequence selected from the group consisting of SEQ ID NO:
- 1 and sequences having at least 95% identity to SEQ ID NO;
1. - View Dependent Claims (22, 23, 27)
- 1 and sequences having at least 95% identity to SEQ ID NO;
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24. An isolated fluorescent polypeptide, the amino acid sequence of which comprises a sequence selected from the group consisting of SEQ ID NO:
- 2 and sequences having at least 95% identity to SEQ ID NO;
2. - View Dependent Claims (25, 26)
- 2 and sequences having at least 95% identity to SEQ ID NO;
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28. A fusion protein comprising a first polypeptide operably linked to a fluorescent polypeptide, the amino acid sequence of the fluorescent polypeptide comprising a sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO;
2, and sequences having at least 95% identity to SEQ ID NO;
1 or SEQ ID NO;
2. - View Dependent Claims (29)
- 1, SEQ ID NO;
Specification