System and Method for Removing a Cryporotectant from a Liquid
First Claim
1. A system for removing cryoprotectant from a cryoprotectant-containing liquid stored a container comprising:
- a dialyzer having a first inlet to receive the cryoprotectant-containing liquid, a first outlet for discharge of the cryoprotectant-liquid, a second inlet to receive a cryoprotectant-free dialysate liquid and a second outlet for discharge of the cryoprotectant-free liquid, said dialyzer forming a diffusion gradient between said cryoprotectant-containing liquid and the cryoprotectant-free liquid;
a source of cryoprotectant-free dialysate liquid capable of receiving cryoprotectant transferred through said diffusion gradient within said dialyzer;
an outlet fluid line connected to said first inlet and connectable to an outlet of the container;
an inlet fluid line connected to said first outlet and connectable to an inlet of the container, said outlet and inlet fluid lines forming a first fluid circuit between the container of the cryoprotectant-containing liquid and said dialyzer;
a second outlet fluid line connected between said source of dialysate liquid and said second inlet;
a discharge fluid line connected to said second outlet and connectable to a waste container, said second outlet fluid line and said discharge fluid line forming a second fluid circuit between said source of dialysate liquid and said dialyzer;
a first pump disposed in said first fluid circuit for controlling the flow of the cryoprotectant-containing liquid through said first fluid circuit;
a second pump disposed in said second fluid circuit for controlling the flow of the dialysate liquid through said second fluid circuit;
a differential conductivity device disposed between said second outlet fluid line and said discharge fluid line and operable to measure the difference in conductivity between dialysate liquid flowing through said second outlet fluid line and dialysate liquid that has received cryoprotectant transferred by said dialyzer flowing through said discharge fluid line;
and a controller for controlling the operation of said first and/or second pump in response to the measured difference in conductivity.
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Accused Products
Abstract
A system for removing cryoprotectant from a cryoprotectant-containing liquid stored a container comprises a cryoprotectant removal device that receives the cryoprotectant-containing liquid and a cryoprotectant-free dialysate liquid and that is operable to transfer cryoprotectant to the dialysate liquid. A differential conductivity device is arranged to continuously measure the difference in conductivity between dialysate liquid entering the device and dialysate liquid that has received cryoprotectant transferred by the dialyzer discharged from the device. A controller is operable to control the flow of the liquids through the device in response to the measured difference in conductivity, and particularly to stop the flow of the cryoprotectant-containing liquid when the measured differential conductivity indicates that the cryoprotectant has been substantially removed from the liquid.
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Citations
13 Claims
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1. A system for removing cryoprotectant from a cryoprotectant-containing liquid stored a container comprising:
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a dialyzer having a first inlet to receive the cryoprotectant-containing liquid, a first outlet for discharge of the cryoprotectant-liquid, a second inlet to receive a cryoprotectant-free dialysate liquid and a second outlet for discharge of the cryoprotectant-free liquid, said dialyzer forming a diffusion gradient between said cryoprotectant-containing liquid and the cryoprotectant-free liquid; a source of cryoprotectant-free dialysate liquid capable of receiving cryoprotectant transferred through said diffusion gradient within said dialyzer; an outlet fluid line connected to said first inlet and connectable to an outlet of the container; an inlet fluid line connected to said first outlet and connectable to an inlet of the container, said outlet and inlet fluid lines forming a first fluid circuit between the container of the cryoprotectant-containing liquid and said dialyzer; a second outlet fluid line connected between said source of dialysate liquid and said second inlet; a discharge fluid line connected to said second outlet and connectable to a waste container, said second outlet fluid line and said discharge fluid line forming a second fluid circuit between said source of dialysate liquid and said dialyzer; a first pump disposed in said first fluid circuit for controlling the flow of the cryoprotectant-containing liquid through said first fluid circuit; a second pump disposed in said second fluid circuit for controlling the flow of the dialysate liquid through said second fluid circuit; a differential conductivity device disposed between said second outlet fluid line and said discharge fluid line and operable to measure the difference in conductivity between dialysate liquid flowing through said second outlet fluid line and dialysate liquid that has received cryoprotectant transferred by said dialyzer flowing through said discharge fluid line; and a controller for controlling the operation of said first and/or second pump in response to the measured difference in conductivity. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method for removing cryoprotectant from a liquid comprising the steps of:
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passing a cryoprotectant-containing liquid and a cryoprotectant-free liquid through a cryoprotectant removal device configured to transfer cryoprotectant from the cryoprotectant-containing liquid to the cryoprotectant-free liquid; measuring the differential conductivity between cryoprotectant-free liquid entering the device and cryoprotectant-free liquid discharged from the device after receiving cryoprotectant transferred within the device; and controlling the flow of the cryoprotectant-containing liquid and/or the cryoprotectant-free liquid through the device in response to the measured differential conductivity. - View Dependent Claims (10, 12, 13)
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Specification