Method and Apparatus for Analysis and Sorting of Polynucleotides Based on Size
First Claim
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1. A method for detecting labeled molecules in a microfluidic device, said method comprisinga) providing labeled molecules, wherein said molecules are labeled with a fluorescent dye;
- b) providing a microfluidic device comprising a microfluidic channel, wherein said microfluidic channel comprises a detection region;
c) transporting the labeled molecules through the microfluidic channel such that the molecules pass though the detection region;
d) illuminating labeled molecules in the detection region with radiation from an excitation light source, wherein said radiation is of a wavelength that excites the fluorescent dye, and wherein the fluorescent dye when excited emits light of characteristic wavelengths; and
e) detecting the emitted light,wherein labeled molecules outside the detection region are not illuminated with radiation from the excitation light source.
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Abstract
The invention relates to a microfabricated device and methods of using the device for analyzing and sorting polynucleotide molecules by size.
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Citations
3 Claims
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1. A method for detecting labeled molecules in a microfluidic device, said method comprising
a) providing labeled molecules, wherein said molecules are labeled with a fluorescent dye; -
b) providing a microfluidic device comprising a microfluidic channel, wherein said microfluidic channel comprises a detection region; c) transporting the labeled molecules through the microfluidic channel such that the molecules pass though the detection region; d) illuminating labeled molecules in the detection region with radiation from an excitation light source, wherein said radiation is of a wavelength that excites the fluorescent dye, and wherein the fluorescent dye when excited emits light of characteristic wavelengths; and e) detecting the emitted light, wherein labeled molecules outside the detection region are not illuminated with radiation from the excitation light source. - View Dependent Claims (2)
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3. A method of sizing polynucleotides in solution, comprising:
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a) flowing a continuous stream of solution containing reporter-labeled polynucleotides through a microfabricated channel comprising a detection region having a selected volume, where the concentration of the molecules in the solution is such that most molecules pass through the detection region one by one, and b) determining the size of each molecule as it passes through the detection region by measuring the level of said reporter.
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Specification