ANTIVIRAL OLIGONUCLEOTIDES TARGETING VIRAL FAMILIES

• US 20100172965A1
• Filed: 12/21/2009
• Published: 07/08/2010
• Est. Priority Date: 09/13/2002
• Status: Abandoned Application

• Current Assignee: Replicor Inc.
• Original Assignee: Replicor Inc.
• Inventors: VAIILANT, Andrew, Juteau, Jean-Marc
• Application Number:
  US12/643,628
• Publication Number:
  US 20100172965A1
• Time in Patent Office: Days
• US Class Current: 424/450
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/7088 Compounds having three or more nucleosides or nucleotides
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C12N 15/11 DNA or RNA fragments; Modified forms thereof DNA or RNA not used in recombinant technology, C07H21/00; Non-coding nucleic acids having a biological activity
• CPC Class Codes: C12N 15/115 Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
• CPC Class Codes: C12N 2310/3125 Methylphosphonates
• CPC Class Codes: C12N 2310/315 Phosphorothioates
• CPC Class Codes: C12N 2310/321 2'-O-R Modification
• CPC Class Codes: C12N 2310/351 Conjugate
• CPC Class Codes: C12N 2310/3521 Methyl

Abstract

Random sequence oligonucleotides that have antiviral activity are described, along with their use as antiviral agents. In many cases, the oligonucleotides are greater than 40 nucleotides in length. Also described are methods for the prophylaxis or treatment of a viral infection in a human or animal, and a method for the prophylaxis treatment of cancer caused by oncoviruses in a human or animal. The methods typically involve administering to a human or animal in need of such treatment, a pharmacologically acceptable, therapeutically effective amount of at least oligonucleotide that does not act by a sequence complementary mode of action.

Claims

1. An antiviral pharmaceutical composition comprising a therapeutically effective amount of at least one antiviral oligonucleotide at least 20 nucleotides in length, wherein said oligonucleotide comprises at least 19 phosphorothioated linkages, does not comprise a CpG motif, does not have a compliment in the influenza A genome sequence and the antiviral activity of said oligonucleotide occurs principally by a non-sequence complimentary mode of action, and said target virus is influenza virus;

and a pharmaceutically acceptable carrier.

2. The antiviral pharmaceutical composition of claim 1, adapted for delivery by oral ingestion.

3. The antiviral pharmaceutical composition of claim 1, adapted for delivery enterally.

4. The antiviral pharmaceutical composition of claim 1, adapted for delivery by injection.

5. The antiviral pharmaceutical composition of claim 1, adapted for delivery by inhalation.

6. The antiviral pharmaceutical composition of claim 1, adapted for delivery topically.

7. The antiviral pharmaceutical composition of claim 1, wherein said composition further comprises a delivery system.

8. The antiviral pharmaceutical composition of claim 1, wherein said composition further comprises a liposomal formulation.

9. The antiviral pharmaceutical composition of claim 1, wherein said composition further comprises at least one other antiviral drug in combination.

10. The antiviral pharmaceutical composition of claim 1, wherein said oligonucleotide is a heteropolymer comprised of two different nucleic acids selected from the group consisting of adenosine, guanosine, cytosine and thymine.

11. The antiviral pharmaceutical composition of claim 1, wherein said oligonucleotide is a heteropolymer comprised of alternating adenosine and cytosine residues.

12. The antiviral pharmaceutical composition of claim 1, wherein said oligonucleotide is SEQ ID NO:

23.

13. The antiviral pharmaceutical composition of claim 1, wherein said oligonucleotide is a heteropolymer comprised of alternating adenosine and guanosine residues.

14. The antiviral pharmaceutical composition of claim 1, wherein said oligonucleotide is SEQ ID NO:

25.

15. The antiviral pharmaceutical composition of any of claims 1 to 14, wherein said oligonucleotide comprises at least one phosphodiester linkage.

16. The antiviral pharmaceutical composition of any of claims 1 to 14, wherein said oligonucleotide comprises at least one modification to its chemical structure.

17. The antiviral pharmaceutical composition of any of claims 1 to 14, wherein said oligonucleotide comprises at least one 2′

modification to the ribose moiety.

18. The antiviral pharmaceutical composition of any of claims 1 to 14, wherein said oligonucleotide comprises at least one 2′

-O methyl modification to the ribose moiety.

19. The antiviral pharmaceutical composition of any of claims 1 to 14, wherein said oligonucleotide comprises at least one 2′

-O (2-methoxyethyl) modification to the ribose moiety.

20. The antiviral pharmaceutical composition of any of claims 1 to 14, wherein said oligonucleotide has all ribose moieties modified with a 2′

modification.

21. The antiviral pharmaceutical composition of any of claims 1 to 14, wherein said oligonucleotide has all ribose moieties modified with a 2′

-O methyl modification.

22. The antiviral pharmaceutical composition of any of claims 1 to 14, wherein said oligonucleotide has all ribose moieties modified with a 2′

-O (2-methoxyethyl) modification.

23. The antiviral pharmaceutical composition of any of claims 1 to 14, wherein said oligonucleotide comprises at least one methylphosphonate linkage.

24. The antiviral pharmaceutical composition of any of claims 1 to 14, wherein said oligonucleotide comprises at least one phosphorodithioated linkage.

25. The antiviral pharmaceutical composition of any of claims 1 to 14, wherein said oligonucleotide comprises at least one locked nucleic acid.

26. The antiviral pharmaceutical composition of any of claims 1 to 14, wherein said oligonucleotide is a concatamer consisting of two or more oligonucleotide sequences joined by a linker.

27. A kit comprising at least one anti-viral oligonucleotide or anti-viral oligonucleotide formulation in a labeled package, wherein said oligonucleotide is at least 20 nucleotides in length, wherein said oligonucleotide comprises at least 19 phosphorothioate linkages, does not comprise a CpG motif, does not have a complement in the influenza A genome, wherein the anti-viral activity of said oligonucleotide occurs principally by a non sequence complementary mode of action, and the label on said package indicates that said anti-viral oligonucleotide can be used against a target virus, and wherein and said target virus is influenza virus.