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Repair of Nucleic Acids for Improved Amplification

  • US 20100173364A1
  • Filed: 04/11/2007
  • Published: 07/08/2010
  • Est. Priority Date: 04/11/2006
  • Status: Abandoned Application
First Claim
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1. A method for repairing a damaged polynucleotide so as to enhance at least one of fidelity and yield of a copied or amplified product of the polynucleotide, comprising:

  • (a) incubating the polynucleotide in a reaction mixture comprising;

    an effective amount of at least one apurinic/apyrimidinic (AP) endonuclease;

    a DNA ligase; and

    at least one of NAD+ or ATP as a cofactor; and

    (b) enhancing at least one of fidelity and yield of the copied or amplified product.

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