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Droplet-based assay system

  • US 20100173394A1
  • Filed: 09/23/2009
  • Published: 07/08/2010
  • Est. Priority Date: 09/23/2008
  • Status: Active Grant
First Claim
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28. A system for sample analysis, comprising:

  • at least one droplet generator that forms a plurality of emulsions including droplets that each contain a sample partition prepared as a reaction mixture for amplification of a nucleic acid target;

    a plate defining an array of cavities to hold the emulsions;

    a heating and cooling device to heat the emulsions disposed in the cavities to induce nucleic acid amplification in droplets;

    a detection assembly to detect signals from intact droplets of the emulsions; and

    a controller in communication with the detection assembly and programmed to estimate a presence, if any, of the nucleic acid target in a sample based on signals detected from the intact droplets.

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