FLUORESCENT QUENCHING DETECTING REAGENTS AND METHODS
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Abstract
Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
37 Citations
48 Claims
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1-31. -31. (canceled)
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32. A modified base comprising a quencher moiety and having the structure:
wherein Ar1 and Ar2 are each independently a substituted or unsubstituted aryl group, R5 is H, (C1-C8)alkyl, aryl, heteroalkyl, or heteroaryl; L is a linking group having from 3 to 100 backbone atoms selected from C, N, O, S, Si and P, said linking group being cyclic, acyclic, aromatic or a combination thereof, and n is an integer from 1 to 10.
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41. A modified base comprising a quencher moiety and having the structure:
wherein Ar1 and Ar2 are each independently a substituted or unsubstituted aryl group, R5 is H, (C1-C8)alkyl, aryl, heteroalkyl, or heteroaryl; L is a linking group having from 3 to 100 backbone atoms selected from C, N, O, S, Si and P, said linking group being cyclic, acyclic, aromatic or a combination thereof, and n is an integer from 1 to 10. - View Dependent Claims (46)
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47. A modified base comprising a quencher moiety and having the structure:
wherein Ar1 and Ar2 are each independently a substituted or unsubstituted aryl group, one of Rx or Ry is dimethoxytrityl and one of Rx or Ry is P(N(iBu)2)(CH2CH2CN), n is an integer from 1 to 10, q is an integer from 1 to 20, R5 is H, (C1-C8)alkyl, aryl, heteroalkyl, or heteroaryl, and L is a linking group having from 3 to 100 backbone atoms selected from C. N, O, S, Si and P, said linking group being cyclic, acyclic, aromatic or a combination thereof. - View Dependent Claims (48)
Specification