METHODS AND KITS FOR DETECTING HEMOGLOBIN IN TEST SAMPLES
First Claim
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1. A method of detecting hemoglobin in a test sample, the method comprising the steps of:
- a) adding at least one basic solution to a test sample;
b) adding an indicator solution to the test sample to generate a light signal, wherein the indicator solution comprises at least one acridinium compound;
wherein steps a) and b) can be performed in any order; and
c) measuring the light generated to detect the hemoglobin in the test sample.
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Abstract
The present invention relates to methods of detecting hemoglobin in a test sample. These methods can be used to diagnose a subject suffering from a genetic disorder relating to hemoglobin metabolism, to determine the eligibility of a subject to be a blood donor, to determine the age of a stored blood sample or to identify a hemolyzed plasma sample. The present invention also relates to kits for use in the above described methods.
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Citations
70 Claims
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1. A method of detecting hemoglobin in a test sample, the method comprising the steps of:
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a) adding at least one basic solution to a test sample; b) adding an indicator solution to the test sample to generate a light signal, wherein the indicator solution comprises at least one acridinium compound; wherein steps a) and b) can be performed in any order; and c) measuring the light generated to detect the hemoglobin in the test sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method of detecting hemoglobin in a test sample, the method comprising the steps of:
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a) adding at least one basic solution to a test sample; and b) measuring the current generated at at least one electrode to detect the hemoglobin in the test sample. - View Dependent Claims (10, 11)
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12. A method of diagnosing a subject suffering from a genetic disorder relating to hemoglobin metabolism, the method comprising the steps of:
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a) adding at least one basic solution to a test sample obtained from a subject suspected of suffering from a genetic disorder relating to hemoglobin metabolism; b) adding an indicator solution to the test sample to generate a light signal, wherein the indicator solution comprises at least one acridinium compound, wherein steps a) and b) can be performed in any order; c) quantifying the light generated to detect the hemoglobin in the test sample; and d) determining the concentration of hemoglobin in the test sample based on the amount of light quantified in step c); and e) comparing the concentration of hemoglobin in step (d) with a predetermined level, wherein if the concentration of hemoglobin determined in step (d) is lower or higher than the predetermined level, then a determination is made that the subject is suffering from a genetic disorder relating to hemoglobin metabolism. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20)
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21. A method of determining the eligibility of a subject to be a blood donor, the method comprising the steps of:
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a) adding at least one basic solution to a test sample obtained from a subject; b) adding an indicator solution to the test sample to generate a light signal, wherein the indicator solution comprises at least one acridinium compound, wherein steps a) and b) can be performed in any order; c) quantifying the light generated to detect the hemoglobin in the test sample; d) determining the concentration of hemoglobin in the test sample based on the amount of light quantified in step c); and e) comparing the concentration of hemoglobin in step (d) with a predetermined level, wherein if the concentration of hemoglobin determined in step (d) is lower or higher than the predetermined level, then a determination is made that the subject is not eligible to be a blood donor. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28)
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29. A method of determining the age of a stored blood sample, the method comprising the steps of:
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a) adding at least one basic solution to a blood sample; b) adding an indicator solution to the blood sample to generate a light signal, wherein the indicator solution comprises at least one acridinium compound, wherein steps a) and b) can be performed in any order; c) quantifying the light generated to detect the hemoglobin in the blood sample; d) determining the concentration of hemoglobin in the blood sample based on the amount of light quantified in step c); and e) comparing the concentration of hemoglobin in step (d) with at least one predetermined level, wherein if the concentration of hemoglobin determined in step (d) is lower then the predetermined level, then the plasma sample is determined to be an older blood sample. - View Dependent Claims (30, 31, 32, 33, 34, 35, 36)
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37. A method of identifying a hemolyzed serum or plasma sample, the method comprising the steps of:
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a) adding at least one basic solution to a serum or plasma sample; b) adding an indicator solution to the serum or plasma sample to generate a light signal, wherein the indicator solution comprises at least one acridinium compound, wherein steps a) and b) can be performed in any order; c) quantifying the light generated to detect the hemoglobin in the serum or plasma sample; d) determining the concentration of hemoglobin in the serum or plasma sample based on the amount of light quantified in step c); and e) comparing the concentration of hemoglobin in step (d) with a predetermined level, wherein if the concentration of hemoglobin determined in step (d) is the same as or higher then the predetermined level, then the serum or plasma sample is determined to be hemolyzed and further wherein if the concentration of hemoglobin determined in step (d) is lower then the predetermined level, then the serum or plasma sample is determined not to be hemolyzed. - View Dependent Claims (38, 39, 40, 41, 42, 43)
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44. A kit for detecting hemoglobin in a test sample, the kit comprising:
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a. at least one basic solution; b. at least one indicator solution, wherein the indicator solution comprises at least one acridinium compound; and c. instructions for detecting hemoglobin in a test sample. - View Dependent Claims (45, 46, 47, 48)
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49. A kit for detecting hemoglobin in a test sample, the kit comprising:
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a. at least one basic solution; b. at least one electrode; and c. instructions for detecting hemoglobin in a test sample. - View Dependent Claims (50)
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51. A kit for diagnosing a subject suffering from a genetic disorder relating to hemoglobin metabolism, the kit comprising:
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a. at least one basic solution; b. at least one indicator solution, wherein the indicator solution comprises at least one acridinium compound; and c. instructions for diagnosing a subject suffering from a genetic disorder relating to hemoglobin metabolism. - View Dependent Claims (52, 53, 54, 55)
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56. A kit for determining the eligibility of a subject to be a blood donor, the kit comprising:
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a. at least one basic solution; b. at least one indicator solution, wherein the indicator solution comprises at least one acridinium compound; and c. instructions for determining the eligibility of a subject to be a blood donor. - View Dependent Claims (57, 58, 59, 60)
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61. A kit for determining the age of a stored blood sample, the kit comprising:
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a. at least one basic solution; b. at least one indicator solution, wherein the indicator solution comprises at least one acridinium compound; and c. instructions for determining the age of a blood sample. - View Dependent Claims (62, 63, 64, 65)
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66. A kit for identifying a hemolyzed serum or plasma sample, the kit comprising:
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a. at least one basic solution; b. at least one indicator solution, wherein the indicator solution comprises at least one acridinium compound; and c. instructions for identifying a hemolyzed serum or plasma sample. - View Dependent Claims (67, 68, 69, 70)
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Specification