PARTICLES FOR USE IN SUPPORTED NUCLEIC ACID LIGATION AND DETECTION SEQUENCING
First Claim
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1. A method of immobilizing a bead to a substrate comprising:
- a.) providing at least one bead with a clean surface;
b.) silylating the bead surface;
c.) reacting the silylated bead surface with at least one poly(ethylene oxide), wherein a hydrated poly(ethylene oxide) bead surface is formed;
d.) providing a functionalized substrate surface; and
e.) reacting the functionalized substrate with the hydrated poly(ethylene oxide) bead surface;
wherein the bead is immobilized to the substrate.
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Abstract
Compositions and methods to modify the surface of particles to which biomolecules are attached are disclosed. The particles can include beads and nanoparticles which are composed of metallics, metal alloys, glass, polymers and derivatives and composites thereof. The surface of the particles are modified to be hydrophilic for ease in the attachment of biomolecules to the particle surface and immobilization of the particles to a substrate to facilitate process such as nucleic acid sequencing, PCR and sequencing by ligation.
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Citations
20 Claims
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1. A method of immobilizing a bead to a substrate comprising:
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a.) providing at least one bead with a clean surface; b.) silylating the bead surface; c.) reacting the silylated bead surface with at least one poly(ethylene oxide), wherein a hydrated poly(ethylene oxide) bead surface is formed; d.) providing a functionalized substrate surface; and e.) reacting the functionalized substrate with the hydrated poly(ethylene oxide) bead surface; wherein the bead is immobilized to the substrate. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of forming a hydrophilic surface on a particle having a plasmon resonance comprising:
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a.) providing at least one substrate particle surface; b.) chemically modifying the surface; and c.) reacting the modified surface with at least one functionalized poly(ethylene oxide); wherein a hydrated poly(ethylene oxide) substrate particle surface is formed. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14)
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15. A method for selectively attaching particles to a substrate surface, the method comprising:
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providing a substrate surface configured to receive a plurality of particles; introducing the plurality of particles onto the substrate surface; and immobilizing the plurality of particles to the substrate surface by a selectively triggered reaction. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification