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PARTICLES FOR USE IN SUPPORTED NUCLEIC ACID LIGATION AND DETECTION SEQUENCING

  • US 20100179075A1
  • Filed: 07/30/2009
  • Published: 07/15/2010
  • Est. Priority Date: 07/30/2008
  • Status: Abandoned Application
First Claim
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1. A method of immobilizing a bead to a substrate comprising:

  • a.) providing at least one bead with a clean surface;

    b.) silylating the bead surface;

    c.) reacting the silylated bead surface with at least one poly(ethylene oxide), wherein a hydrated poly(ethylene oxide) bead surface is formed;

    d.) providing a functionalized substrate surface; and

    e.) reacting the functionalized substrate with the hydrated poly(ethylene oxide) bead surface;

    wherein the bead is immobilized to the substrate.

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