METHODS FOR INCREASING TRICHOGENICITY OF DERMAL CELLS

• US 20100179492A1
• Filed: 01/14/2009
• Published: 07/15/2010
• Est. Priority Date: 01/14/2009
• Status: Abandoned Application

• Current Assignee: Aderans Research Institute Incorporated
• Original Assignee: Aderans Research Institute Incorporated
• Inventors: Ensslin, Michael, Sergott, Luke, Nace, Arben, Zheng, Ying, Homan, Ying, Stenn, Kurt
• Application Number:
  US12/353,699
• Publication Number:
  US 20100179492A1
• Time in Patent Office: Days
• US Class Current: 604/290
• CPC Class Codes: A61B 18/203 applying laser energy to the outside of the body
• CPC Class Codes: A61B 2018/00452 Skin
• CPC Class Codes: A61K 35/36 Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells islets of Langerhans A61K35/39

Abstract

Methods for increasing trichogenic activity of populations of dermal cells by inducing local trauma to skin tissue that serves as a source of the dermal cells are provided. Methods for obtaining dermal cells with increased trichogenic activity and for using the disclosed dermal cells to implant into a mammalian host at a site of desired follicle generation are also provided.

Claims

1. A method for increasing the trichogenic ability of a population of dermal cells comprising inducing local trauma to a section of skin comprising dermal cells.

2. The method of claim 1, wherein the local trauma is induced by mechanical or chemical means.

3. The method of claim 2, wherein the local trauma is induced by hair shaft plucking.

4. The method of claim 2, wherein the local trauma is induced by scratching or abrasion of the skin.

5. The method of claim 2, wherein the local trauma is induced by irritating the skin with lasers, hyperthermia, or caustic chemicals selected from the group consisting of retinoic acid or cyclosporine derivatives.

6. A method for obtaining an isolated population of dermal cells for use in hair transplants comprising:

a) inducing local trauma to a section of skin tissue from which the population of dermal cells is derived;

b) obtaining the area of skin tissue subjected to the local trauma; and

c) dissociating the skin tissue into cells,

wherein the primary cells include a population of dermal cells that have an increased trichogenic ability relative to a population of dermal cells dissociated from skin tissue that is not subjected to local trauma.

7. The method of claim 6, wherein the local trauma is induced by mechanical or chemical means.

8. The method of claim 7, wherein the local trauma is induced by hair shaft plucking.

9. The method of claim 7, wherein the local trauma is induced by scratching or abrasion of the skin.

10. The method of claim 7, wherein the local trauma is induced by irritating the skin with lasers, hyperthermia, or caustic chemicals selected from the group consisting of retinoic acid or cyclosporine derivatives.

11. The method of claim 6, wherein the area of skin subjected to the local trauma is obtained after between 30 minutes and 4 weeks, 30 minutes and 180 minutes, 30 minutes and 120 minutes, or 30 minutes and 60 minutes following induction of the local trauma.

12. The method of claim 11, wherein the area of skin subjected to the local trauma is obtained after between 30 and 60 minutes following induction of the local trauma.

13. An isolated population of dermal cells obtained using the method of claim 6, wherein the population of dermal cells has an increased trichogenic ability relative to a population of dermal cells dissociated from skin tissue that is not subjected to local trauma.

20. A system for generating new hair follicles in a mammalian subject comprising:

a) an isolated population of dermal cells according to claim 13; and

b) a means for implanting the dermal cells, and optionally, additional cells into the dermis or subcutis of a mammalian host at a site of desired hair growth.

14. A method for generating new hair follicles in a mammalian subject comprising:

a) inducing local trauma to a section of skin tissue of the mammal;

b) obtaining the area of skin tissue subjected to the local trauma;

c) dissociating the skin tissue into populations of dermal cells and epidermal cells,

wherein the dermal cells have an increased trichogenic ability relative to a population of dermal cells dissociated from skin tissue that is not subjected to local trauma; and

d) implanting the dermal cells into the dermis or subcutis of a mammalian host at a site of desired hair growth.

15. The method of claim 14, wherein the local trauma is induced by mechanical or chemical means.

16. The method of claim 15, wherein the local trauma is induced by hair shaft plucking.

17. The method of claim 15, wherein the local trauma is induced by scratching or abrasion of the skin.

18. The method of claim 15, wherein the local trauma is induced by irritating the skin with caustic chemicals, retinoic acid or cyclosporine derivatives.

19. The method of claim 14 further comprising combining the dermal cells with epidermal cells and implanting the combined dermal and epidermal cells into the dermis or subcutis of a mammalian host at a site of desired hair growth.