Detection of Herpes Simplex Virus

• US 20100184018A1
• Filed: 01/26/2010
• Published: 07/22/2010
• Est. Priority Date: 01/31/2001
• Status: Active Grant

• Current Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic)
• Original Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic)
• Inventors: Smith, Thomas F., Wold, Arlo, Uhl, Jim, Espy, Mark J.
• Granted Patent: US 7,893,241 B2
• Time in Patent Office: Days
• US Class Current: 435/5
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 1/705 for herpetoviridae, e.g. herpes simplex, varicella zoster

Abstract

The invention provides methods to detect herpes simplex virus (HSV) in biological samples and further to distinguish between HSV-1 and HSV-2. Primers and probes for the differential detection of HSV-1 and HSV-2 are provided by the invention. Articles of manufacture containing such primers and probes for detecting HSV are further provided by the invention.

Claims

1. An article of manufacture comprising:

a pair of HSV TK primers, wherein said pair of HSV TK primers comprise a first HSV TK primer and a second HSV TK primer, wherein said first HSV TK primer consists of the sequence 5′

-CAC GCT RCT GCG GGT TTA TAT AGA-3′

(SEQ ID NO;

7), wherein R is A or G, and wherein said second HSV TK primer consists of the sequence 5′

-TTG TTA TCT GGG CGC TMG TCA TT-3′

(SEQ ID NO;

8), wherein M is A or C;

a pair of HSV TK probes, wherein said pair of HSV TK probes comprise a first HSV TK probe and a second HSV TK probe, wherein said first HSV TK probe consists of the sequence 5′

-CGC GCG ACG ATA TCG TCT ACG TAC-3′

(SEQ ID NO;

9), and wherein said second HSV TK probe consists of the sequence 5′

-CGA GCC GAT GAC TTA CTG GCA GGT G-3′

(SEQ ID NO;

10); and

a donor fluorescent moiety and a corresponding acceptor moiety.

2. The article of manufacture of claim 1, wherein said pair of HSV TK probes are labeled with said donor fluorescent moiety and said acceptor fluorescent moiety.

3. The article of manufacture of claim 1, further comprising a label or package insert having instructions thereon for using said pair of HSV TK primers and said pair of TK HSV probes to detect the presence or absence of HSV in a biological sample.

4. The article of manufacture of 1, further comprising a label or package insert having instructions thereon for using said pair of HSV TK probes to distinguish between HSV-1 and HSV-2.