PROBE, PROBE SET, PROBE CARRIER, AND TESTING METHOD
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Abstract
A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 3 and mutated sequences thereof.
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Citations
11 Claims
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1-7. -7. (canceled)
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8. A method of specifically detecting a DNA of Candida tropicalis which is a pathogenic fungus, comprising:
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(i) reacting a sample with a probe carrier comprising at least two of the following probes (A) to (L), which are immobilized on a carrier as arranged at intervals, under stringent conditions; and (ii) detecting a reaction intensity of each probe on the probe carrier reacted with a nucleic acid contained in the sample to detect the DNA of Candida tropicalis that reacts with all of the at least two probes; (A) a probe consisting essentially of a base sequence represented by accgccagaggttataactaaaccaaa (SEQ ID NO.
1);(B) a probe consisting essentially of a base sequence represented by gagcaatacgctaggtttgtttgaaagaa (SEQ ID NO.
2);(C) a probe consisting essentially of a base sequence represented by acgcttattttgctagtggccacc (SEQ ID NO.
3);(D) a probe consisting essentially of a complementary sequence of SEQ ID NO. 1; (E) a probe consisting essentially of a complementary sequence of SEQ ID NO. 2; (F) a probe consisting essentially of a complementary sequence of SEQ ID NO. 3; (G) a probe a mutated sequence obtained by deletion, substitution or addition of a base on SEQ ID NO. 1 within a range that the mutated sequence retains a function as a probe for detecting a DNA of Candida tropicalis;
(H) a probe consisting essentially of a mutated sequence obtained by deletion, substitution or addition of a base on SEQ ID NO. 2 within a range that the mutated sequence retains the function as a probe for detecting a DNA of Candida tropicalis;
(I) a probe consisting essentially of a mutated sequence obtained by deletion, substitution or addition of a base on SEQ ID NO. 3 within a range that the mutated sequence retains the function as a probe for detecting a DNA of Candida tropicalis;
(J) a probe consisting essentially of a mutated sequence obtained by deletion, substitution or addition of a base on the complementary sequence of SEQ ID NO. 1 within a range that the mutated sequence retains the function as a probe for detecting a DNA of Candida tropicalis;
(K) a probe consisting essentially of a mutated sequence obtained by deletion, substitution or addition of a base on the complementary sequence of SEQ ID NO. 2 within a range that the mutated sequence retains the function as a probe for detecting a DNA of Candida tropicalis; and (L) a probe consisting essentially of a mutated sequence obtained by deletion, substitution or addition of a base on the complementary sequence of SEQ ID NO. 3 within a range that the mutated sequence retains the function as a probe for detecting a DNA of Candida tropicalis. - View Dependent Claims (9, 10, 11)
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Specification