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SOLUBLE ANALYTE DETECTION AND AMPLIFICATION

  • US 20100184076A1
  • Filed: 02/12/2010
  • Published: 07/22/2010
  • Est. Priority Date: 09/02/2003
  • Status: Active Grant
First Claim
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1. A method for detecting a compound of interest in a sample comprising the steps of:

  • a) providing a binding construct comprising a recognition portion which recognizes and binds said compound of interest, and a nucleic acid portion;

    b) mixing said binding construct with said sample to form construct-compound complexes in solution;

    c) providing one or more surfaces, wherein said surfaces are magnetic particles bearing one or more accessible binding targets capable of recognizing and binding to said recognition portion of said binding construct;

    d) introducing said one or more surfaces to said solution of said binding construct and said sample in order for said one or more surfaces to form construct-surface complexes with any unbound binding constructs;

    e) separating said construct-surface complexes from said solution leaving behind said construct-compound complexes in solution without physical removal of the construct-surface complexes from the solution; and

    f) detecting the presence or absence of said nucleic acid portion of said binding construct in the solution,wherein the presence of said nucleic acid portion of said binding construct indicates the presence of said compound of interest in said sample.

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